• Product name

    Anti-SMURF 2 antibody [EP629Y3]
    See all SMURF 2 primary antibodies
  • Description

    Rabbit monoclonal [EP629Y3] to SMURF 2
  • Host species

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SMURF 2 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • SH-SY-5Y cell lysate
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab53316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
    • Tissue specificity

      Widely expressed.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Contains 1 C2 domain.
      Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
      Contains 3 WW domains.
    • Domain

      The second and third WW domains are responsible for interaction with the PY-motif of R-SMAD (SMAD1, SMAD2 and SMAD3).
      The C2 domain is involved in autoinhibition of the catalytic activity by interacting with the HECT domain.
    • Post-translational

      Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1.
    • Cellular localization

      Nucleus. Cytoplasm. Cell membrane. Membrane raft. Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.
    • Information by UniProt
    • Database links

    • Alternative names

      • E3 ubiquitin-protein ligase SMURF2 antibody
      • EC 6.3.2. antibody
      • hSMURF2 antibody
      • MGC138150 antibody
      • Smad specific E3 ubiquitin ligase 2 antibody
      • SMAD specific E3 ubiquitin protein ligase 2 antibody
      • SMAD ubiquitination regulatory factor 2 antibody
      • SMAD-specific E3 ubiquitin-protein ligase 2 antibody
      • SMUF2_HUMAN antibody
      • Smurf2 antibody
      • Ubiquitin protein ligase SMURF2 antibody
      see all


    • Anti-SMURF 2 antibody [EP629Y3] (ab53316) at 1/500 dilution + SH-SY-5Y cell lysate at 10 µg

      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 86 kDa
      Observed band size: 86 kDa


    This product has been referenced in:

    • Mund T & Pelham HR Substrate clustering potently regulates the activity of WW-HECT domain-containing ubiquitin ligases. J Biol Chem 293:5200-5209 (2018). Read more (PubMed: 29463679) »
    • Huang PS  et al. The Long Non-Coding RNA MIR503HG Enhances Proliferation of Human ALK-Negative Anaplastic Large-Cell Lymphoma. Int J Mol Sci 19:N/A (2018). WB . Read more (PubMed: 29758012) »
    See all 9 Publications for this product

    Customer reviews and Q&As

    Western blot
    Mouse Cell lysate - whole cell (mouse liver cell line)
    Loading amount
    15 µg
    mouse liver cell line
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Dr. Frank Götschel

    Verified customer

    Submitted Sep 09 2009

    For licensing inquiries, please contact partnerships@abcam.com

    Sign up