Recombinant
Anti-SMURF 2 antibody [EP629Y3] (ab53316)
- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameAnti-SMURF 2 antibody [EP629Y3]
See all SMURF 2 primary antibodies -
DescriptionRabbit monoclonal [EP629Y3] to SMURF 2
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human SMURF 2 aa 200-300. The exact sequence is proprietary.
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Positive control
- SH-SY-5Y cell lysate
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP629Y3
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab53316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
Target
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FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
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Tissue specificityWidely expressed.
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PathwayProtein modification; protein ubiquitination.
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Sequence similaritiesContains 1 C2 domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 3 WW domains. -
DomainThe second and third WW domains are responsible for interaction with the PY-motif of R-SMAD (SMAD1, SMAD2 and SMAD3).
The C2 domain is involved in autoinhibition of the catalytic activity by interacting with the HECT domain. -
Post-translational
modificationsAuto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1. -
Cellular localizationNucleus. Cytoplasm. Cell membrane. Membrane raft. Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.
- Information by UniProt
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Database links
- Entrez Gene: 64750 Human
- Entrez Gene: 66313 Mouse
- Entrez Gene: 303614 Rat
- Omim: 605532 Human
- SwissProt: Q9HAU4 Human
- SwissProt: A2A5Z6 Mouse
- Unigene: 340955 Mouse
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Alternative names
- E3 ubiquitin-protein ligase SMURF2 antibody
- EC 6.3.2. antibody
- hSMURF2 antibody
see all
Images
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![Western blot - Anti-SMURF 2 antibody [EP629Y3] (ab53316)](https://www.abcam.com/ps/products/53/ab53316/Images/smurf.png)
Western blot - Anti-SMURF 2 antibody [EP629Y3] (ab53316)Anti-SMURF 2 antibody [EP629Y3] (ab53316) at 1/500 dilution + SH-SY-5Y cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDa
References
This product has been referenced in:
- Mund T & Pelham HR Substrate clustering potently regulates the activity of WW-HECT domain-containing ubiquitin ligases. J Biol Chem 293:5200-5209 (2018). Read more (PubMed: 29463679) »
- Huang PS et al. The Long Non-Coding RNA MIR503HG Enhances Proliferation of Human ALK-Negative Anaplastic Large-Cell Lymphoma. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29758012) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"