Recombinant
RabMAb

Recombinant Anti-SMURF 2 antibody [EP629Y3] - BSA and Azide free (ab239855)

Overview

  • Product name

    Anti-SMURF 2 antibody [EP629Y3] - BSA and Azide free
    See all SMURF 2 primary antibodies
  • Description

    Rabbit monoclonal [EP629Y3] to SMURF 2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SMURF 2 aa 200-300. The exact sequence is proprietary.

  • General notes

    Ab239855 is the carrier-free version of ab53316. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239855 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
    • Tissue specificity

      Widely expressed.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Contains 1 C2 domain.
      Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
      Contains 3 WW domains.
    • Domain

      The second and third WW domains are responsible for interaction with the PY-motif of R-SMAD (SMAD1, SMAD2 and SMAD3).
      The C2 domain is involved in autoinhibition of the catalytic activity by interacting with the HECT domain.
    • Post-translational
      modifications

      Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1.
    • Cellular localization

      Nucleus. Cytoplasm. Cell membrane. Membrane raft. Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.
    • Information by UniProt
    • Database links

    • Alternative names

      • E3 ubiquitin-protein ligase SMURF2 antibody
      • EC 6.3.2. antibody
      • hSMURF2 antibody
      • MGC138150 antibody
      • Smad specific E3 ubiquitin ligase 2 antibody
      • SMAD specific E3 ubiquitin protein ligase 2 antibody
      • SMAD ubiquitination regulatory factor 2 antibody
      • SMAD-specific E3 ubiquitin-protein ligase 2 antibody
      • SMUF2_HUMAN antibody
      • Smurf2 antibody
      • Ubiquitin protein ligase SMURF2 antibody
      see all

    References

    ab239855 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab239855.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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