Overview

  • Product name

  • Description

    Rabbit polyclonal to SMURF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human SMURF1 aa 198-374.
    Database link: Q9HCE7

  • Positive control

    • WB: Mouse brain and liver tissue lysate. IHC-P: Human kidney and testis tissue. ICC/IF: HeLa and HepG2 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000.
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Acts by mediating ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 2 WW domains.
  • Post-translational
    modifications

    Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin-protein ligase SMURF1 antibody
    • hSMURF1 antibody
    • KIAA1625 antibody
    • Smad specific E3 ubiquitin ligase 1 antibody
    • SMAD specific E3 ubiquitin protein ligase 1 antibody
    • Smad ubiquitination regulatory factor 1 antibody
    • SMAD-specific E3 ubiquitin-protein ligase 1 antibody
    • SMUF1_HUMAN antibody
    • SMURF 1 antibody
    • smurf1 antibody
    see all

Images

  • All lanes : Anti-SMURF1 antibody (ab236081) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse brain tissue lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
  • HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for SMURF1 using ab236081 at 1/100 dilution in ICC/IF. An Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody.

  • Paraffin-embedded human kidney testis stained for SMURF1 using ab236081 at 1/100 dilution in immunohistochemical analysis.

  • HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for SMURF1 using ab236081 at 1/100 dilution in ICC/IF. An Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody.

  • Paraffin-embedded human kidney tissue stained for SMURF1 using ab236081 at 1/100 dilution in immunohistochemical analysis.

References

ab236081 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab236081.
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