Overview

  • Product name

  • Description

    Rabbit polyclonal to SMURF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human SMURF1 aa 150-250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab107712)

  • Positive control

    • This antibody gave a positive signal in Human bone tumour tissue lysate as well as the following whole cell lysates: HeLa; U20S; MG-63; Saos2.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).

Target

  • Function

    E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Acts by mediating ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 2 WW domains.
  • Post-translational
    modifications

    Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin-protein ligase SMURF1 antibody
    • hSMURF1 antibody
    • KIAA1625 antibody
    • Smad specific E3 ubiquitin ligase 1 antibody
    • SMAD specific E3 ubiquitin protein ligase 1 antibody
    • Smad ubiquitination regulatory factor 1 antibody
    • SMAD-specific E3 ubiquitin-protein ligase 1 antibody
    • SMUF1_HUMAN antibody
    • SMURF 1 antibody
    • smurf1 antibody
    see all

Images

  • All lanes : Anti-SMURF1 antibody (ab94480) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 3 : MG-63 (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 4 : Human bone tumor tissue lysate - total protein (ab29359)
    Lane 5 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 86 kDa
    Observed band size: 86 kDa
    Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes

References

ab94480 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100

Dr. Kirk Mcmanus

Verified customer

Submitted May 05 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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