Key features and details
- Rabbit polyclonal to SMYD1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-SMYD1 antibody
See all SMYD1 primary antibodies
DescriptionRabbit polyclonal to SMYD1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment, corresponding to a region within amino acids 118-374 of Human SMYD1 (Q8NB12).
- 293T and A431 whole cell lysates; Rat RT2 xenograft and Human HBL435 xenograft tissues; HeLa cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab127570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Predicted molecular weight: 56 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionActs as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis.
Sequence similaritiesContains 1 MYND-type zinc finger.
Contains 1 SET domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- BOP antibody
- CD8 beta opposite antibody
- CD8b opposite antibody
All lanes : Anti-SMYD1 antibody (ab127570) at 1/10000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 56 kDa
7.5% SDS PAGE
ab127570 at 1/500 dilution staining SMYD1 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Alpha tubulin filaments are stained red.
ab127570 at 1/500 dilution staining SMYD1 in paraffin-embedded Rat RT2 xenograft tissue by Immunohistochemistry.
ab127570 at 1/500 dilution staining SMYD1 in paraffin-embedded Human HBL435 xenograft tissue by Immunohistochemistry.
ab127570 has not yet been referenced specifically in any publications.