Overview

  • Product name
  • Description
    Rabbit polyclonal to SMYD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Cow, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SMYD1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab34471.)

  • Positive control
    • This antibody gave a positive result in human Skeletal Muscle Tissue Lysate (adult normal tissue). IF/ICC: HeLa cell line.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Target

Images

  • ICC/IF image of ab34472 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34472, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Just S  et al. The myosin-interacting protein SMYD1 is essential for sarcomere organization. J Cell Sci 124:3127-36 (2011). IHC ; Zebrafish . Read more (PubMed: 21852424) »
  • Sirinupong N  et al. Crystal Structure of Cardiac-specific Histone Methyltransferase SmyD1 Reveals Unusual Active Site Architecture. J Biol Chem 285:40635-44 (2010). WB . Read more (PubMed: 20943667) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your interest in our SMYD1 antibodies, and for bringing the zebrafish article to our attention. Unfortunately, I do not see an order from any of the instituions listed in the author affilitaions section. We also have not removed any SMYD1 antibodies from our catalogue, so it must have been one of those three. My first guess was that they used ab32482, the antibody that includes some IHC data from one of our collaborators in the reviews section of the datasheet. But the immunogen has little homology with Danio Smyd1a or Smyd1b. http://www.uniprot.org/uniprot/Q2MJQ9 http://www.uniprot.org/uniprot/Q2MJQ8 The immunogen for antibody ab34472, on the other hand, has 72% identity with each. I am not sure which of these to recommend but ab34472 is more likely to cross-react. I am getting alignment results for the third, ab49327, and will send the results when I recieve them.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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