Anti-SMYD3 antibody (ab155018)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-SMYD3 antibody
See all SMYD3 primary antibodies -
DescriptionRabbit polyclonal to SMYD3
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment corresponding to a region within amino acids 22-411 of Human SMYD3.
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Positive control
- 293T and HeLa whole cell lysates; HeLa cells; Human hepatoma tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab155018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 49 kDa. | |
| IP | 1/500 - 1/1000. | |
| IHC-P | 1/100 - 1/1000. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionHistone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
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Tissue specificityExpressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.
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Sequence similaritiesBelongs to the histone-lysine methyltransferase family.
Contains 1 MYND-type zinc finger.
Contains 1 SET domain. -
Cellular localizationCytoplasm. Nucleus. Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.
- Information by UniProt
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Database links
- Entrez Gene: 64754 Human
- Omim: 608783 Human
- SwissProt: Q9H7B4 Human
- Unigene: 567571 Human
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Alternative names
- bA74P14.1 (novel protein) antibody
- bA74P14.1 antibody
- FLJ21080 antibody
see all
Images
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Western blot - Anti-SMYD3 antibody (ab155018)Anti-SMYD3 antibody (ab155018) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 49 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody (ab155018)Confocal immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling SMYD3 with ab155018 at 1/500 dilution (green). Alpha-tubulin filaments were labeled with an Alpha-tubulin antibody at 1/2000 dilution (red). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody (ab155018)Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling SMYD3 with ab155018 at 1/500 dilution. -
Immunoprecipitation - Anti-SMYD3 antibody (ab155018)ab155018 immunoprecipitates SMYD3 protein in IP experiments.IP Sample: 1000 µg HeLa whole cell lysate
Lane 1. Control with 2.5 µg of preimmune rabbit IgG
Lane 2. Immunoprecipitation of SMYD3 protein using 2.5 µg of ab155018.
The immunoprecipitated SMYD3 protein was detected using ab155018 at 1/1000 dilution.
12% SDS-PAGE.
Protocols
References
ab155018 has not yet been referenced specifically in any publications.
