Anti-SMYD3 antibody (ab155018)
Key features and details
- Rabbit polyclonal to SMYD3
- Suitable for: WB, IP, ICC/IF, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMYD3 antibody
See all SMYD3 primary antibodies -
Description
Rabbit polyclonal to SMYD3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IF, ChIPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 22-411 of Human SMYD3.
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Positive control
- Chip: HepG2 chromatin extract; WB: A431, A549, SK-OV-3, HeLa, HepG2, HCT 116 and HEK-293T lysate. IF: HCT 116 whole cell.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab155018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 49 kDa. | |
| IP | 1/100 - 1/500. | |
| ICC/IF | 1/100 - 1/1000. | |
| ChIP | Use at an assay dependent concentration. |
Target
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Function
Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. -
Tissue specificity
Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas. -
Sequence similarities
Belongs to the histone-lysine methyltransferase family.
Contains 1 MYND-type zinc finger.
Contains 1 SET domain. -
Cellular localization
Cytoplasm. Nucleus. Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M. - Information by UniProt
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Database links
- Entrez Gene: 64754 Human
- Omim: 608783 Human
- SwissProt: Q9H7B4 Human
- Unigene: 567571 Human
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Alternative names
- bA74P14.1 (novel protein) antibody
- bA74P14.1 antibody
- FLJ21080 antibody
see all
Images
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ChIP - Anti-SMYD3 antibody (ab155018)
Cross-linked ChIP was performed with HepG2 (human liver hepatocellular carcinoma cell line) chromatin extract and 5 μg of either normal rabbit IgG or ab155018. The precipitated DNA was detected by PCR with primer set targeting to 5' flanking region of Nkx2.8..
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Western blot - Anti-SMYD3 antibody (ab155018)All lanes : Anti-SMYD3 antibody (ab155018) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : SK-OV-3 (human ovarian cancer cell line) whole cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 49 kDa10% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody (ab155018)Immunofluorescence analysis of ice-cold MeOH-fixed HCT 116 (human colorectal carcinoma cell line) whole cell labeling SMYD3 protein at cytoplasm (green)with ab155018 at 1/500 dilution. Blue: Hoechst 33342 staining.
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Immunoprecipitation - Anti-SMYD3 antibody (ab155018)Immunoprecipitation of SMYD3 protein from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate.
IP antibody:
1- Control IgG;
2- ab155018, 5 µg.
Western blot analysis was performed using ab155018 at 1/500 dilution, followed by anti-Rabbit IgG antibody. -
Western blot - Anti-SMYD3 antibody (ab155018)All lanes : Anti-SMYD3 antibody (ab155018) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 49 kDa10% SDS-PAGE
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Western blot - Anti-SMYD3 antibody (ab155018)All lanes :
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 49 kDa10% SDS-PAGE
Protocols
References (1)
ab155018 has been referenced in 1 publication.
- Stefanowicz D et al. Epigenetic modifying enzyme expression in asthmatic airway epithelial cells and fibroblasts. BMC Pulm Med 17:24 (2017). PubMed: 28137284
