Overview

  • Product name
    Anti-SMYD3 antibody
    See all SMYD3 primary antibodies
  • Description
    Rabbit polyclonal to SMYD3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 22-411 of Human SMYD3.

  • Positive control
    • 293T and HeLa whole cell lysates; HeLa cells; Human hepatoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IP 1/500 - 1/1000.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
  • Tissue specificity
    Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.
  • Sequence similarities
    Belongs to the histone-lysine methyltransferase family.
    Contains 1 MYND-type zinc finger.
    Contains 1 SET domain.
  • Cellular localization
    Cytoplasm. Nucleus. Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.
  • Information by UniProt
  • Database links
    • Alternative names
      • bA74P14.1 (novel protein) antibody
      • bA74P14.1 antibody
      • FLJ21080 antibody
      • histone lysine N methyltransferase SMYD3 antibody
      • KMT3E antibody
      • MGC104324 antibody
      • SET and MYND domain containing 3 antibody
      • SET and MYND domain containing protein 3 antibody
      • SET and MYND domain-containing protein 3 antibody
      • SMYD 3 antibody
      • Smyd3 antibody
      • SMYD3 protein antibody
      • SMYD3_HUMAN antibody
      • Zinc finger MYND domain containing 1 antibody
      • Zinc finger MYND domain containing protein 1 antibody
      • Zinc finger MYND domain-containing protein 1 antibody
      • Zinc finger protein subfamily 3A MYND domain containing 1 antibody
      • Zinc finger protein, subfamily 3A (MYND domain containing), 1 antibody
      • ZMYND 1 antibody
      • ZMYND1 antibody
      • ZNFN3A1 antibody
      see all

    Images

    • Western blot - Anti-SMYD3 antibody (ab155018)
      Western blot - Anti-SMYD3 antibody (ab155018)
      Anti-SMYD3 antibody (ab155018) at 1/1000 dilution + 293T whole cell lysate at 30 µg

      Predicted band size: 49 kDa



      10% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody (ab155018)
      Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody (ab155018)
      Confocal immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling SMYD3 with ab155018 at 1/500 dilution (green). Alpha-tubulin filaments were labeled with an Alpha-tubulin antibody at 1/2000 dilution (red).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody (ab155018)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody (ab155018)
      Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling SMYD3 with ab155018 at 1/500 dilution.
    • Immunoprecipitation - Anti-SMYD3 antibody (ab155018)
      Immunoprecipitation - Anti-SMYD3 antibody (ab155018)
      ab155018 immunoprecipitates SMYD3 protein in IP experiments.

      IP Sample: 1000 µg HeLa whole cell lysate
      Lane 1. Control with 2.5 µg of preimmune rabbit IgG
      Lane 2. Immunoprecipitation of SMYD3 protein using 2.5 µg of ab155018.
      The immunoprecipitated SMYD3 protein was detected using ab155018 at 1/1000 dilution.
      12% SDS-PAGE.

    References

    ab155018 has not yet been referenced specifically in any publications.

    Publishing research using ab155018? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab155018.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up