For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    smyd3-antibody-ab155018.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation
Share by email

Anti-SMYD3 antibody (ab155018)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

ChIP - Anti-SMYD3 antibody (ab155018)
  • Western blot - Anti-SMYD3 antibody (ab155018)
  • Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody (ab155018)
  • Immunoprecipitation - Anti-SMYD3 antibody (ab155018)
  • Western blot - Anti-SMYD3 antibody (ab155018)
  • Western blot - Anti-SMYD3 antibody (ab155018)

Key features and details

  • Rabbit polyclonal to SMYD3
  • Suitable for: WB, IP, ICC/IF, ChIP
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human SMYD3 protein (ab125590)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-SMYD3 antibody
    See all SMYD3 primary antibodies
  • Description

    Rabbit polyclonal to SMYD3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IF, ChIPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 22-411 of Human SMYD3.

  • Positive control

    • Chip: HepG2 chromatin extract; WB: A431, A549, SK-OV-3, HeLa, HepG2, HCT 116 and HEK-293T lysate. IF: HCT 116 whole cell.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Other
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Lysine methylation
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human SMYD3 protein (ab125590)
  • Related Products

    • Recombinant Human SMYD3 protein (ab125590)

Applications

Our Abpromise guarantee covers the use of ab155018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IP 1/100 - 1/500.
ICC/IF 1/100 - 1/1000.
ChIP Use at an assay dependent concentration.

Target

  • Function

    Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
  • Tissue specificity

    Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.
  • Sequence similarities

    Belongs to the histone-lysine methyltransferase family.
    Contains 1 MYND-type zinc finger.
    Contains 1 SET domain.
  • Cellular localization

    Cytoplasm. Nucleus. Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.
  • Target information above from: UniProt accession Q9H7B4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 64754 Human
    • Omim: 608783 Human
    • SwissProt: Q9H7B4 Human
    • Unigene: 567571 Human
    • Alternative names

      • bA74P14.1 (novel protein) antibody
      • bA74P14.1 antibody
      • FLJ21080 antibody
      • histone lysine N methyltransferase SMYD3 antibody
      • KMT3E antibody
      • MGC104324 antibody
      • SET and MYND domain containing 3 antibody
      • SET and MYND domain containing protein 3 antibody
      • SET and MYND domain-containing protein 3 antibody
      • SMYD 3 antibody
      • Smyd3 antibody
      • SMYD3 protein antibody
      • SMYD3_HUMAN antibody
      • Zinc finger MYND domain containing 1 antibody
      • Zinc finger MYND domain containing protein 1 antibody
      • Zinc finger MYND domain-containing protein 1 antibody
      • Zinc finger protein subfamily 3A MYND domain containing 1 antibody
      • Zinc finger protein, subfamily 3A (MYND domain containing), 1 antibody
      • ZMYND 1 antibody
      • ZMYND1 antibody
      • ZNFN3A1 antibody
      see all

    Images

    • ChIP - Anti-SMYD3 antibody (ab155018)
      ChIP - Anti-SMYD3 antibody (ab155018)

      Cross-linked ChIP was performed with HepG2 (human liver hepatocellular carcinoma cell line) chromatin extract and 5 μg of either normal rabbit IgG or ab155018. The precipitated DNA was detected by PCR with primer set targeting to 5' flanking region of Nkx2.8..

    • Western blot - Anti-SMYD3 antibody (ab155018)
      Western blot - Anti-SMYD3 antibody (ab155018)
      All lanes : Anti-SMYD3 antibody (ab155018) at 1/1000 dilution

      Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
      Lane 2 : SK-OV-3 (human ovarian cancer cell line) whole cell lysate
      Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG antibody

      Predicted band size: 49 kDa



      10% SDS-PAGE

    • Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody (ab155018)
      Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody (ab155018)

      Immunofluorescence analysis of ice-cold MeOH-fixed HCT 116 (human colorectal carcinoma cell line) whole cell labeling SMYD3 protein at cytoplasm (green)with ab155018 at 1/500 dilution. Blue: Hoechst 33342 staining.

    • Immunoprecipitation - Anti-SMYD3 antibody (ab155018)
      Immunoprecipitation - Anti-SMYD3 antibody (ab155018)

      Immunoprecipitation of SMYD3 protein from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate.

      IP antibody:

      1- Control IgG;

      2- ab155018, 5 µg.


      Western blot analysis was performed using ab155018 at 1/500 dilution, followed by anti-Rabbit IgG antibody.

    • Western blot - Anti-SMYD3 antibody (ab155018)
      Western blot - Anti-SMYD3 antibody (ab155018)
      All lanes : Anti-SMYD3 antibody (ab155018) at 1/1000 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
      Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 49 kDa



      10% SDS-PAGE

    • Western blot - Anti-SMYD3 antibody (ab155018)
      Western blot - Anti-SMYD3 antibody (ab155018)
      All lanes :

      Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : Transfected HEK-293T whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG antibody

      Predicted band size: 49 kDa



      10% SDS-PAGE

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab155018? Please let us know so that we can cite the reference in this datasheet.

    ab155018 has been referenced in 1 publication.

    • Stefanowicz D  et al. Epigenetic modifying enzyme expression in asthmatic airway epithelial cells and fibroblasts. BMC Pulm Med 17:24 (2017). PubMed: 28137284

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab155018.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.