Key features and details
- Rabbit polyclonal to SMYD3
- Suitable for: IP, ChIP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SMYD3 antibody
See all SMYD3 primary antibodies
DescriptionRabbit polyclonal to SMYD3
Tested applicationsSuitable for: IP, ChIP, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Pig, Rhesus monkey
Recombinant fragment within Human SMYD3 (internal sequence). The exact sequence is proprietary.
Database link: Q9H7B4
- WB: A549 whole cell lysate. IHC-P: Human colon carcinoma tissue. IP: HEK-293T whole cell lysate. ChIP: HepG2 chromatin extract.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab228015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/100 - 1/500.|
|ChIP||Use at an assay dependent concentration.|
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 49 kDa.|
FunctionHistone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
Tissue specificityExpressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family.
Contains 1 MYND-type zinc finger.
Contains 1 SET domain.
Cellular localizationCytoplasm. Nucleus. Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.
- Information by UniProt
- bA74P14.1 (novel protein) antibody
- bA74P14.1 antibody
- FLJ21080 antibody
Anti-SMYD3 antibody (ab228015) at 1/1000 dilution + A549 (human lung carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 49 kDa
10% SDS-PAGE gel.
Cross-linked ChIP was performed with HepG2 (human liver hepatocellular carcinoma cell line) chromatin extract and 5 μg of either control rabbit IgG or ab228015. The precipitated DNA was detected by PCR with primer set targeting to 5' flanking region of Nkx2.8.
Paraffin-embedded human colon cancer tissue stained for SMYD3 using ab228015 at 1/250 dilution in immunohistochemical analysis.
SMYD3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 2.5 µg of ab228015. Western blot was performed from the immunoprecipitate using ab228015 at 1/1000 dilution. Anti-rabbit IgG (HRP) was used as a secondary reagent.
Lane 1: 2.5 µg preimmune rabbit IgG instaed of ab228015 in HEK-293T whole cell lysate.
Lane 2: ab228015 IP in HEK-293T whole cell lysate.
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228015 has not yet been referenced specifically in any publications.