Overview

  • Product name

  • Description

    Rabbit polyclonal to SMYD3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, ChIP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human SMYD3 (internal sequence). The exact sequence is proprietary.
    Database link: Q9H7B4

  • Positive control

    • WB: A549 whole cell lysate. IHC-P: Human colon carcinoma tissue. IP: HEK-293T whole cell lysate. ChIP: HepG2 chromatin extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab228015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100 - 1/500.
ChIP Use at an assay dependent concentration.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.

Target

  • Function

    Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
  • Tissue specificity

    Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.
  • Sequence similarities

    Belongs to the histone-lysine methyltransferase family.
    Contains 1 MYND-type zinc finger.
    Contains 1 SET domain.
  • Cellular localization

    Cytoplasm. Nucleus. Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.
  • Information by UniProt
  • Database links

  • Alternative names

    • bA74P14.1 (novel protein) antibody
    • bA74P14.1 antibody
    • FLJ21080 antibody
    • histone lysine N methyltransferase SMYD3 antibody
    • KMT3E antibody
    • MGC104324 antibody
    • SET and MYND domain containing 3 antibody
    • SET and MYND domain containing protein 3 antibody
    • SET and MYND domain-containing protein 3 antibody
    • SMYD 3 antibody
    • Smyd3 antibody
    • SMYD3 protein antibody
    • SMYD3_HUMAN antibody
    • Zinc finger MYND domain containing 1 antibody
    • Zinc finger MYND domain containing protein 1 antibody
    • Zinc finger MYND domain-containing protein 1 antibody
    • Zinc finger protein subfamily 3A MYND domain containing 1 antibody
    • Zinc finger protein, subfamily 3A (MYND domain containing), 1 antibody
    • ZMYND 1 antibody
    • ZMYND1 antibody
    • ZNFN3A1 antibody
    see all

Images

  • Anti-SMYD3 antibody (ab228015) at 1/1000 dilution + A549 (human lung carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 49 kDa



    10% SDS-PAGE gel.

  • Cross-linked ChIP was performed with HepG2 (human liver hepatocellular carcinoma cell line) chromatin extract and 5 μg of either control rabbit IgG or ab228015. The precipitated DNA was detected by PCR with primer set targeting to 5' flanking region of Nkx2.8.

  • Paraffin-embedded human colon cancer tissue stained for SMYD3 using ab228015 at 1/250 dilution in immunohistochemical analysis.

  • SMYD3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 2.5 µg of ab228015. Western blot was performed from the immunoprecipitate using ab228015 at 1/1000 dilution. Anti-rabbit IgG (HRP) was used as a secondary reagent.

    Lane 1: 2.5 µg preimmune rabbit IgG instaed of ab228015 in HEK-293T whole cell lysate. 

    Lane 2: ab228015 IP in HEK-293T whole cell lysate. 

    10% SDS-PAGE gel.

References

ab228015 has not yet been referenced specifically in any publications.

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