Recombinant
RabMAb

Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)

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Overview

  • Product name
    Anti-SMYD3 antibody [EPR11106(2)] - N-terminal
    See all SMYD3 primary antibodies
  • Description
    Rabbit monoclonal [EPR11106(2)] to SMYD3 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SMYD3 aa 1-100. The exact sequence is proprietary.
    Database link: Q9H7B4

  • Positive control
    • HeLa, 293, MCF-7 and T47-D cell lysates, Human testis and skeletal muscle tissue, HeLa cells, 293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).
ICC/IF 1/500.
IP 1/40 - 1/60.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function
    Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
  • Tissue specificity
    Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.
  • Sequence similarities
    Belongs to the histone-lysine methyltransferase family.
    Contains 1 MYND-type zinc finger.
    Contains 1 SET domain.
  • Cellular localization
    Cytoplasm. Nucleus. Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.
  • Information by UniProt
  • Database links
    • Alternative names
      • bA74P14.1 (novel protein) antibody
      • bA74P14.1 antibody
      • FLJ21080 antibody
      • histone lysine N methyltransferase SMYD3 antibody
      • KMT3E antibody
      • MGC104324 antibody
      • SET and MYND domain containing 3 antibody
      • SET and MYND domain containing protein 3 antibody
      • SET and MYND domain-containing protein 3 antibody
      • SMYD 3 antibody
      • Smyd3 antibody
      • SMYD3 protein antibody
      • SMYD3_HUMAN antibody
      • Zinc finger MYND domain containing 1 antibody
      • Zinc finger MYND domain containing protein 1 antibody
      • Zinc finger MYND domain-containing protein 1 antibody
      • Zinc finger protein subfamily 3A MYND domain containing 1 antibody
      • Zinc finger protein, subfamily 3A (MYND domain containing), 1 antibody
      • ZMYND 1 antibody
      • ZMYND1 antibody
      • ZNFN3A1 antibody
      see all

    Images

    • Western blot - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)
      Western blot - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)
      All lanes : Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498) at 1/10000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : 293 lysate
      Lane 3 : T47-D lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 49 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)

      Immunohistochemical analysis of paraffin-embedded Human skeletal muscle labeling SMYD3 at 1/100 dilution and HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

    • Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)
      Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)

      Flow cytometric analysis of HeLa cells fixed in 20 °C acetone labeling SMYD3 at 1/500 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 (red). Counterstained with DAPI (blue)

    • Immunoprecipitation - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)
      Immunoprecipitation - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)

      Immunoprecipitation of HeLa cells labeling SMYD3 with ab183498 at 1/50 dilution and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.  

    • Flow Cytometry - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)
      Flow Cytometry - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)

      Flow cytometric analysis of 293 cells fixed in 2% paraformaldehyde labeling SMYD3 with ab183498 at 1/110 dilution and Goat anti rabbit IgG (FITC) at 1/150 dilution. Rabbit Monoclonal IgG was used as an isotype control.

    • Western blot - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)
      Western blot - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)
      Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498) at 1/2000 dilution + MCF-7 lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 49 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)

      Immunohistochemical analysis of paraffin-embedded Human testis labeling SMYD3 with ab183498 at 1/100 dilution and  HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

    References

    ab183498 has not yet been referenced specifically in any publications.

    Publishing research using ab183498? Please let us know so that we can cite the reference in this datasheet.

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