Overview

  • Product name

    Anti-SMYD3 antibody [EPR11107(2)]
    See all SMYD3 primary antibodies
  • Description

    Rabbit monoclonal [EPR11107(2)] to SMYD3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SMYD3 aa 250-350 (internal sequence). The exact sequence is proprietary.
    Database link: Q9H7B4

  • Positive control

    • WB: Wild-type HAP1, MCF7, HeLa, HEK-293, T47-D and NIH/3T3 cell lysates; Mouse brain and Rat spleen tissue lysates. IHC-P: Human colonic carcinoma and mouse skeletal muscle tissues. ICC/IF: MCF7 and HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab187149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/40 - 1/60.

Target

  • Function

    Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
  • Tissue specificity

    Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.
  • Sequence similarities

    Belongs to the histone-lysine methyltransferase family.
    Contains 1 MYND-type zinc finger.
    Contains 1 SET domain.
  • Cellular localization

    Cytoplasm. Nucleus. Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.
  • Information by UniProt
  • Database links

  • Alternative names

    • bA74P14.1 (novel protein) antibody
    • bA74P14.1 antibody
    • FLJ21080 antibody
    • histone lysine N methyltransferase SMYD3 antibody
    • KMT3E antibody
    • MGC104324 antibody
    • SET and MYND domain containing 3 antibody
    • SET and MYND domain containing protein 3 antibody
    • SET and MYND domain-containing protein 3 antibody
    • SMYD 3 antibody
    • Smyd3 antibody
    • SMYD3 protein antibody
    • SMYD3_HUMAN antibody
    • Zinc finger MYND domain containing 1 antibody
    • Zinc finger MYND domain containing protein 1 antibody
    • Zinc finger MYND domain-containing protein 1 antibody
    • Zinc finger protein subfamily 3A MYND domain containing 1 antibody
    • Zinc finger protein, subfamily 3A (MYND domain containing), 1 antibody
    • ZMYND 1 antibody
    • ZMYND1 antibody
    • ZNFN3A1 antibody
    see all

Images

  • All lanes : Anti-SMYD3 antibody [EPR11107(2)] (ab187149) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : SMYD3 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 49 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab187149 observed at 49 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab187149 was shown to react with SMYD3 in HAP1 wild-type cells in Western blot. Loss of signal was observed when SMYD3 knockout sample was used. HAP1 wild-type and SMYD3 knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab187149 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-SMYD3 antibody [EPR11107(2)] (ab187149) at 1/20000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 4 : T47-D (human ductal breast epithelial tumor cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 49 kDa



    Blocking and dilution buffer: 5% NFDM/TBST

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells, labeling SMYD3 with ab187149 at 1/250 dilution (left image; green); secondary antibody was Goat anti rabbit IgG Alexa Fluor®488, and counterstained with Dapi (right image; blue).

  • Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue, labeling SMYD3 with ab187149 at 1/100 dilution. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemical analysis of 2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling SMYD3 with ab187149 at 1/30 dilution. Secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control was a Rabbit monoclonal IgG.

  • Immunoprecipitation analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate labeling SMYD3 using ab187149 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of HeLa lysate.

  • All lanes : Anti-SMYD3 antibody [EPR11107(2)] (ab187149) at 1/5000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat spleen tissue lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 49 kDa



    Blocking and dilution buffer: 5% NFDM/TBST

  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue, labeling SMYD3 with ab187149 at 1/100 dilution. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Ma SJ  et al. Correlations ofEZH2andSMYD3gene polymorphisms with breast cancer susceptibility and prognosis. Biosci Rep 38:N/A (2018). WB ; Human . Read more (PubMed: 29089464) »
  • Ting HA  et al. Notch ligand Delta-like 4 induces epigenetic regulation of Treg cell differentiation and function in viral infection. Mucosal Immunol 11:1524-1536 (2018). Read more (PubMed: 30038214) »
See all 5 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab187149.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up