Overview

  • Product name

    Anti-SMYD5 antibody - C-terminal
    See all SMYD5 primary antibodies
  • Description

    Rabbit polyclonal to SMYD5 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 305-401 of Human SMYD5.

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji cell lysates; NCI-N87 xenograft tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-SMYD5 antibody - C-terminal (ab137622) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 47 kDa



    10% SDS PAGE
  • ab137622 at a 1/100 dilution, staining SMYD5 in paraffin embedded NCI-N87 xenograft tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Liu T  et al. Master redox regulator Trx1 upregulates SMYD1 & modulates lysine methylation. Biochim Biophys Acta 1854:1816-22 (2015). WB ; Human . Read more (PubMed: 26410624) »
See 1 Publication for this product

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