Key features and details
- Rabbit polyclonal to SMYD5 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SMYD5 antibody - C-terminal
See all SMYD5 primary antibodies
DescriptionRabbit polyclonal to SMYD5 - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Synthetic peptide, corresponding to a region within C terminal amino acids 305-401 of Human SMYD5.
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji cell lysates; NCI-N87 xenograft tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 47 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesContains 1 MYND-type zinc finger.
Contains 1 SET domain.
- Information by UniProt
- Protein NN8 4AG antibody
- Protein NN8-4AG antibody
- Retinoic acid induced protein 15 antibody
All lanes : Anti-SMYD5 antibody - C-terminal (ab137622) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa
10% SDS PAGE
ab137622 at a 1/100 dilution, staining SMYD5 in paraffin embedded NCI-N87 xenograft tissue by Immunohistochemistry.
ab137622 has been referenced in 1 publication.
- Liu T et al. Master redox regulator Trx1 upregulates SMYD1 & modulates lysine methylation. Biochim Biophys Acta 1854:1816-22 (2015). WB ; Human . PubMed: 26410624