Overview

  • Product name

    SN 38, DNA topoisomerase I inhibitor
  • Description

    DNA topoisomerase I inhibitor. Active metabolite of CPT-11.
  • Alternative names

    • 7-Ethyl-10-hydroxycamptothecin
  • Biological description

    Active metabolite of CPT-11 (Irinotecan) (ab141107). Inhibits DNA topoisomerase I (IC50 values are 0.74 and 1.9 μM in P388 and Ehrlich cells respectively) and DNA and RNA synthesis (IC50 values are 77 nM and 1.3 μM respectively). Shows potent antitumor activity, causes S- and G2 cell cycle arrest and induces apoptosis. Active in vivo.
  • Purity

    > 99%
  • CAS Number

    86639-52-3
  • Chemical structure

    Chemical Structure

Properties

Applications

Our Abpromise guarantee covers the use of ab141108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab2893 staining γH2A.X in HeLa cells treated with SN 38 (ab141108), by ICC/IF. Increase of γH2A.X nuclear expression correlates with increased concentration of SN 38, as described in literature.
    The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab141108 (SN 38) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2893 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:

  • Maurya DK  et al. Topoisomerase I inhibitor SN-38 effectively attenuates growth of human non-small cell lung cancer cell lines in vitro and in vivo. J Environ Pathol Toxicol Oncol 30:1-10 (2011). Read more (PubMed: 21609311) »
  • Koizumi F  et al. Novel SN-38-incorporating polymeric micelles, NK012, eradicate vascular endothelial growth factor-secreting bulky tumors. Cancer Res 66:10048-56 (2006). Read more (PubMed: 17047068) »
  • Kawato Y  et al. Intracellular roles of SN-38, a metabolite of the camptothecin derivative CPT-11, in the antitumor effect of CPT-11. Cancer Res 51:4187-91 (1991). Read more (PubMed: 1651156) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab141108.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
For licensing inquiries, please contact partnerships@abcam.com

Sign up