Overview

  • Product name

  • Description

    Rabbit polyclonal to SNAI3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Cow
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MPRSFLVKTH SSHRVPNYGK LETLREANGS CSACKELAGS RHLPDEEAPC) of Mouse SNAI3 (NP_038942).

  • Positive control

    • Mouse uterus lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab105604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Seems to inhibit myoblast differentiation. Transcriptional repressor of E-box-dependent transactivation of downstream myogenic bHLHs genes. Binds preferentially to the canonical E-box sequences 5'-CAGGTG-3' and 5'-CACCTG-3'.
  • Sequence similarities

    Belongs to the snail C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • Domain

    Binds E-box via C2H2-type zinc finger domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC129606 antibody
    • Protein snail homolog 3 antibody
    • SMUC antibody
    • SNAI3 antibody
    • SNAI3_HUMAN antibody
    • Snail homolog 3 (Drosophila) antibody
    • SNAIL3 antibody
    • Zfp293 antibody
    • Zinc finger protein 293 antibody
    • Zinc finger protein SNAI3 antibody
    • ZNF293 antibody
    see all

Images

  • Anti-SNAI3 antibody (ab105604) at 1 µg/ml + Mouse uterus lysate at 10 µg

    Predicted band size: 32 kDa



    Gel concentration: 12%

References

ab105604 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

Thank you for your reply. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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Answer

Thank you for your reply. Yes, I have requested that our online datasheet be updated with the correct lysate information. Also, the lab did indicate that no further clearing of the uterine lysate was performed. This antibody was very clean in this particular sample. Regarding a replacement, I can send out another vial from a different lot or offer a different antibody. Unfortunately, I was unable to find another SNAI3 antibody in our catalog. Is there another antibody you might be interested in receiving? I look forward to your reply so that I may assist you further.

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Answer

Thank you for your phone call regarding ab10504. I have contacted the laboratory regarding WB with his antibody. Regarding our image, there are two bands on the western blot shown on the datasheet. A light band at ~32kD and a darker band at ~42kD that is presumed to be a non-specific band. ~30ug of mouse uterus lysate was run for this western blot. We have not run thymus lysates, but have found the mouse uterus lysate to be very clean and show few background bands. As for suggestions, I can suggest loading more protein on the gel could help and being sure that the samples are sufficiently reduced. A purified protein as a positive control would be ideal, however, working with minigels shouldn't be a problem and I see no need to switch to a larger gel. If anything, running a higher percentage gel to improve the resolution of the bands would be useful. If you would prefer, I'd be happy to offer a replacement or a credit rather than continue to troubleshoot with this antibody. Please let me know which you would prefer.

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