Anti-SNAI3 antibody (ab154408)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-SNAI3 antibody
See all SNAI3 primary antibodies -
Description
Rabbit polyclonal to SNAI3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 1-217 of Human SNAI3 (UniProt ID: Q3KNW1).
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Positive control
- A549 whole cell lysate; A549 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 32 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Seems to inhibit myoblast differentiation. Transcriptional repressor of E-box-dependent transactivation of downstream myogenic bHLHs genes. Binds preferentially to the canonical E-box sequences 5'-CAGGTG-3' and 5'-CACCTG-3'. -
Sequence similarities
Belongs to the snail C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
Domain
Binds E-box via C2H2-type zinc finger domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 333929 Human
- Omim: 612741 Human
- SwissProt: Q3KNW1 Human
- Unigene: 499548 Human
- Unigene: 673548 Human
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Alternative names
- MGC129606 antibody
- Protein snail homolog 3 antibody
- SMUC antibody
see all
Images
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Western blot - Anti-SNAI3 antibody (ab154408)Anti-SNAI3 antibody (ab154408) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 32 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-SNAI3 antibody (ab154408)Immunofluorescence analysis of paraformaldehyde-fixed A549 cells, labeling SNAI3 using ab154408 at a 1/500 dilution.
The image in the lower panel was co-stained with Hoechst 33342.
References
ab154408 has not yet been referenced specifically in any publications.
