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  1. Link

    snail--slug-antibody-ab167609.pdf

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Neuroscience Cell Type Marker Neural Stem Cell marker
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Anti-SNAIL + SLUG antibody (ab167609)

  • Datasheet
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Western blot - Anti-SNAIL + SLUG antibody (ab167609)
  • Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG antibody (ab167609)
  • Western blot - Anti-SNAIL + SLUG antibody (ab167609)

Key features and details

  • Mouse polyclonal to SNAIL + SLUG
  • Suitable for: WB, ICC/IF
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-SNAIL antibody [EPR21043] (ab216347)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-SNAIL + SLUG antibody
    See all SNAIL + SLUG primary antibodies
  • Description

    Mouse polyclonal to SNAIL + SLUG
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Recombinant full length protein aa 1-264. Corresponding to Human SNAIL.
    Sequence:

    MPRSFLVRKPSDPNRKPNYSELQDSNPEFTFQQPYDQAHLLAAIPPPEIL NPTASLPMLIWDSVLAPQAQPIAWASLRLQESPRVAELTSLSDEDSGKGS QPPSPPSPAPSSFSSTSVSSLEAEAYAAFPGLGQVPKQLAQLSEAKDLQA RKAFNCKYCNKEYLSLGALKMHIRSHTLPCVCGTCGKAFSRPWLLQGHVR THTGEKPFSCPHCSRAFADRSNLRAHLQTHSDVKKYQCQACARTFSRMSL LHKHQESGCSGCPR


    Database link: NP_005976.2
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • SNAIl transfected 293T cell lysate. HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.4
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Stem Cells
    • Neural Stem Cells
    • Neural Crest Stem Cells
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Developmental Biology
    • Organogenesis
    • Nervous system development

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)
  • Related Products

    • Recombinant Human SNAIL protein (ab134870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab167609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
ICC/IF
Use a concentration of 10 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
ICC/IF
Use a concentration of 10 µg/ml.

Target

  • Relevance

    Function: SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. PTM: SNAIL is phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Similarity: Both SNAIL and SLUG belong to the snail C2H2-type zinc-finger protein family. Tissue specificity: SNAIL is expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. SLUG is expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
  • Cellular localization

    Slug is generally nuclear, while Snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
  • Database links

    • Entrez Gene: 6591 Human
    • Entrez Gene: 6615 Human
    • Omim: 604238 Human
    • SwissProt: O43623 Human
    • SwissProt: O95863 Human
    • Alternative names

      • dJ710H13.1 antibody
      • MGC10182 antibody
      • Neural crest transcription factor Slug antibody
      • Protein sna antibody
      • Protein snail homolog 1 antibody
      • Protein snail homolog 2 antibody
      • Protein snail homolog antibody
      • Slug homolog zinc finger protein antibody
      • Slug zinc finger protein antibody
      • SLUGH antibody
      • SLUGH 1 antibody
      • SLUGH1 antibody
      • SLUGH2 antibody
      • SNA antibody
      • Sna protein antibody
      • SNAH antibody
      • SNAI 2 antibody
      • snai1 antibody
      • SNAI1_HUMAN antibody
      • Snai2 antibody
      • SNAI2_HUMAN antibody
      • Snail 2 antibody
      • Snail homolog 1 (Drosophila) antibody
      • Snail homolog 2 antibody
      • Snail2 antibody
      • WS 2D antibody
      • WS2D antibody
      • Zinc finger protein SLUG antibody
      • Zinc finger protein SNAI1 antibody
      • Zinc finger protein SNAI2 antibody
      see all

    Images

    • Western blot - Anti-SNAIL + SLUG antibody (ab167609)
      Western blot - Anti-SNAIL + SLUG antibody (ab167609)
      All lanes : Anti-SNAIL + SLUG antibody (ab167609) at 1 µg/ml

      Lane 1 : SNAIL transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 15 µl per lane.

      Secondary
      All lanes : Goat anti-Mouse IgG

      Predicted band size: 29 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG antibody (ab167609)
      Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG antibody (ab167609)
      Immunofluorescent analysis of HeLa cells labeling SNAIL with ab167609 at 10 µg/ml.
    • Western blot - Anti-SNAIL + SLUG antibody (ab167609)
      Western blot - Anti-SNAIL + SLUG antibody (ab167609)
      Lane 1 : Monoclonal Mouse anti-GST at 1 µg
      Lanes 2 & 5 : Milk at 5 %
      Lane 3 : Anti-SNAIL + SLUG antibody (ab167609) at 1 µg
      Lane 4 : Monoclonal Mouse anti-GST at 1 µg/ml
      Lane 6 : Anti-SNAIL + SLUG antibody (ab167609) at 1 µg/ml

      Lanes 1-3 : SNAIL-GST fusion protein
      Lanes 4-6 : SLUG-GST-fusion protein

      Lysates/proteins at 0.2 µg per lane.

      Secondary
      All lanes : HRP conjugated Goat anti-mouse at 1/5000 dilution

      Predicted band size: 29 kDa
      Additional bands at: 55.5 kDa (possible tagged protein), 56.4 kDa (possible tagged protein)


      Exposure time: 90 seconds


      Substrate: Perkin Elmer

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (15)

    Publishing research using ab167609? Please let us know so that we can cite the reference in this datasheet.

    ab167609 has been referenced in 15 publications.

    • He X  et al. Oestrogen induces epithelial-mesenchymal transition in endometriosis via circ_0004712/miR-148a-3p sponge function. J Cell Mol Med 24:9658-9666 (2020). PubMed: 32667746
    • Xiong W  et al. E2 -mediated EMT by activation of ß-catenin/Snail signalling during the development of ovarian endometriosis. J Cell Mol Med 23:8035-8045 (2019). PubMed: 31560827
    • Zhao W  et al. MicroRNA-153 suppresses cell invasion by targeting SNAI1 and predicts patient prognosis in glioma. Oncol Lett 17:1189-1195 (2019). PubMed: 30655883
    • Yan P  et al. Tamoxifen attenuates dialysate-induced peritoneal fibrosis by inhibiting GSK-3ß/ß-catenin axis activation. Biosci Rep 38:N/A (2018). PubMed: 30061174
    • Li Q  et al. HSCs-derived COMP drives hepatocellular carcinoma progression by activating MEK/ERK and PI3K/AKT signaling pathways. J Exp Clin Cancer Res 37:231 (2018). PubMed: 30231922
    View all Publications for this product

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab167609.
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