Overview

  • Product name

    Anti-SNAIL + SLUG antibody [CL3700] - N-terminal
    See all SNAIL + SLUG primary antibodies
  • Description

    Mouse monoclonal [CL3700] to SNAIL + SLUG - N-terminal
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment aa 1-28 (N terminal). Corresponding to Human SNAIL.
    Sequence:

    MPRSFLVRKPSDPNRKPNYSELQDSNPE


    Database link: O95863

  • Positive control

    • IHC-P: Human colorectal cancer, breast cancer, fallopian tube, colon, testis, cortex and prostate tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab224731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/5000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Function: SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. PTM: SNAIL is phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Similarity: Both SNAIL and SLUG belong to the snail C2H2-type zinc-finger protein family. Tissue specificity: SNAIL is expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. SLUG is expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
  • Cellular localization

    Slug is generally nuclear, while Snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
  • Database links

  • Alternative names

    • dJ710H13.1 antibody
    • MGC10182 antibody
    • Neural crest transcription factor Slug antibody
    • Protein sna antibody
    • Protein snail homolog 1 antibody
    • Protein snail homolog 2 antibody
    • Protein snail homolog antibody
    • Slug homolog zinc finger protein antibody
    • Slug zinc finger protein antibody
    • SLUGH antibody
    • SLUGH 1 antibody
    • SLUGH1 antibody
    • SLUGH2 antibody
    • SNA antibody
    • Sna protein antibody
    • SNAH antibody
    • SNAI 2 antibody
    • snai1 antibody
    • SNAI1_HUMAN antibody
    • Snai2 antibody
    • SNAI2_HUMAN antibody
    • Snail 2 antibody
    • Snail homolog 1 (Drosophila) antibody
    • Snail homolog 2 antibody
    • Snail2 antibody
    • WS 2D antibody
    • WS2D antibody
    • Zinc finger protein SLUG antibody
    • Zinc finger protein SNAI1 antibody
    • Zinc finger protein SNAI2 antibody
    see all

Images

  • Paraffin-embedded human colorectal cancer tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.

  • Paraffin-embedded human breast cancer tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.

  • Paraffin-embedded human fallopian tube tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.

  • Paraffin-embedded human colon tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.

  • Paraffin-embedded human testis tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.

  • Paraffin-embedded human cortex tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.

  • Paraffin-embedded human prostate tissue stained for SNAIL using ab224731 at 1/5000 dilution in immunohistochemical analysis.

References

ab224731 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab224731.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up