Product nameAnti-SNAIL antibody
See all SNAIL primary antibodies
DescriptionGoat polyclonal to SNAIL
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: Rat kidney lysate, A431 lysate. This antibody gave a positive signal when tested against the SNAIL recombinant protein. IHC-P: Human kidney
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab53519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml.
Primary incubation 1 hour at room temperature.
|IHC-P||Use a concentration of 5 µg/ml.
Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
FunctionInvolved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.
Tissue specificityExpressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.
Sequence similaritiesBelongs to the snail C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers.
modificationsPhosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.
Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2.
O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.
ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation.
Cellular localizationNucleus. Cytoplasm. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
- Information by UniProt
- dJ710H13.1 antibody
- Protein sna antibody
- Protein snail homolog 1 antibody
Western blot of Rat Kidney lysate (35µg protein in RIPA buffer) staining SNAIL with ab53519 at 0.1µg/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
All lanes : Anti-SNAIL antibody (ab53519) at 1/500 dilution
Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
Lane 2 : SLUG (SNAI2) Human Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 68 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes
Ab53519 recognizes the tagged recombinant SNAIL protein which has an expected molecular weight of 68 kDa.
Primary incubation 1 hour at room temperature.
Immunohistochemical analysis of paraformaldehyde fixed mouse tissue sections, of an urethane induced lung adenocarcinoma, labelling SNAIL in the nuclei and cytoplasm. ab53519 was used at a 1/1000 dilution in 4% donkey serum for 16 hours at 4°C. Hear mediated antigen retrieval with Citrate pH6. Blocking with 4% serum for 2 hours at 25°C. Secondary antibody was a Biotinylated Anti-Goat IgG (H+L).
This product has been referenced in:
- Cai X et al. miR-124a enhances therapeutic effects of bone marrow stromal cells transplant on diabetic nephropathy-related epithelial-to-mesenchymal transition and fibrosis. J Cell Biochem 121:299-312 (2020). Read more (PubMed: 31190436) »
- Tian Y et al. Extracellular Hsp90a and clusterin synergistically promote breast cancer epithelial-to-mesenchymal transition and metastasis via LRP1. J Cell Sci 132:N/A (2019). Read more (PubMed: 31273033) »