Recombinant Anti-SNAIL antibody [EPR21043] (ab216347)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21043] to SNAIL
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SNAIL antibody [EPR21043]
See all SNAIL primary antibodies -
Description
Rabbit monoclonal [EPR21043] to SNAIL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: His-tagged human SNAIL recombinant protein (aa1-264); HeLa and HCT 116 whole cell lysates. IP: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21043 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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ELISA pair antibody
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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IP |
1/30.
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Notes |
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WB
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
IP
1/30. |
Target
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Function
Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself. -
Tissue specificity
Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. -
Sequence similarities
Belongs to the snail C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers. -
Post-translational
modificationsPhosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.
Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2.
O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.
ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation. -
Cellular localization
Nucleus. Cytoplasm. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs. - Information by UniProt
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Database links
- Entrez Gene: 6615 Human
- Omim: 604238 Human
- SwissProt: O95863 Human
- Unigene: 48029 Human
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Alternative names
- dJ710H13.1 antibody
- Protein sna antibody
- Protein snail homolog 1 antibody
see all
Images
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All lanes : Anti-SNAIL antibody [EPR21043] (ab216347) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SNAI1 CRISPR-Cas9 edited HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-SNAIL antibody [EPR21043] staining at 1/500 dilution, shown in green; loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) staining at 1/20000 dilution, shown in red. In Western blot, ab216347 was shown to bind specifically to SNAIL. A band was observed at 33 kDa in wild-type HeLa cell lysates with no signal observed at this size in SNAI1 CRISPR-Cas9 edited cell line ab265963 (CRISPR-Cas9 edited cell lysate ab257692). The band observed in the CRISPR-Cas9 edited lysate lane below 33 kDa is likely to represent a truncated form of SNAIL. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and SNAI1 CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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SNAIL was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab216347 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216347 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1: HeLa whole cell lysate 10 μg (Input).
Lane 2: ab216347 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216347 in HeLa whole cell lysate.Exposure time: 10 seconds.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-SNAIL antibody [EPR21043] (ab216347) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
SNAIL expression is not detectable in MCF7 cells, which is consistent with what has been described in the literature (PMID: 10655587 and 22028892).
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All lanes : Anti-SNAIL antibody [EPR21043] (ab216347) at 1/10000 dilution
Lane 1 : His-tagged human SNAIL recombinant protein (aa1-264)
Lane 2 : His-tagged human Slug recombinant protein (aa21-268)
Lane 3 : His-tagged human Slug recombinant protein (aa1-110)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (82)
ab216347 has been referenced in 82 publications.
- Gong H et al. E2F transcription factor 1 (E2F1) enhances the proliferation, invasion and EMT of trophoblast cells by binding to Zinc Finger E-Box Binding Homeobox 1 (ZEB1). Bioengineered 13:2360-2370 (2022). WB ; Human . PubMed: 35030974
- Qiu F et al. Circ_0000658 knockdown inhibits epithelial-mesenchymal transition in bladder cancer via miR-498-induced HMGA2 downregulation. J Exp Clin Cancer Res 41:22 (2022). WB ; Human . PubMed: 35031054
- Xiang J et al. NEK2 enhances malignancies of glioblastoma via NIK/NF-?B pathway. Cell Death Dis 13:58 (2022). WB ; Human . PubMed: 35031599
- Wang J et al. Circular RNA circCSPP1 promotes the occurrence and development of colon cancer by sponging miR-431 and regulating ROCK1 and ZEB1. J Transl Med 20:58 (2022). WB ; Human . PubMed: 35101080
- Han D et al. Dynamic assembly of the mRNA m6A methyltransferase complex is regulated by METTL3 phase separation. PLoS Biol 20:e3001535 (2022). WB ; Human . PubMed: 35143475