Recombinant Anti-SNAIL antibody [EPR21043] (ab216347)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21043] to SNAIL
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-SNAIL antibody [EPR21043]
See all SNAIL primary antibodies -
Description
Rabbit monoclonal [EPR21043] to SNAIL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human SNAIL aa 1 to the C-terminus.
Database link: O95863 -
Positive control
- WB: His-tagged human SNAIL recombinant protein (aa1-264); HeLa and HCT 116 whole cell lysates. IP: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21043 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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ELISA pair antibody
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab216347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). | |
| IP | 1/30. |
Target
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Function
Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself. -
Tissue specificity
Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. -
Sequence similarities
Belongs to the snail C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers. -
Post-translational
modificationsPhosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.
Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2.
O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.
ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation. -
Cellular localization
Nucleus. Cytoplasm. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs. - Information by UniProt
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Database links
- Entrez Gene: 6615 Human
- Omim: 604238 Human
- SwissProt: O95863 Human
- Unigene: 48029 Human
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Alternative names
- dJ710H13.1 antibody
- Protein sna antibody
- Protein snail homolog 1 antibody
see all
Images
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![Immunoprecipitation - Anti-SNAIL antibody [EPR21043] (ab216347)](https://www.abcam.com/ps/products/216/ab216347/Images/ab216347-296143-anti-snail-antibody-epr21043-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SNAIL antibody [EPR21043] (ab216347)SNAIL was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab216347 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216347 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1: HeLa whole cell lysate 10 μg (Input).
Lane 2: ab216347 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216347 in HeLa whole cell lysate.Exposure time: 10 seconds.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-SNAIL antibody [EPR21043] (ab216347)](https://www.abcam.com/ps/products/216/ab216347/Images/ab216347-296142-anti-snail-antibody-epr21043-western-blot.jpg)
Western blot - Anti-SNAIL antibody [EPR21043] (ab216347)All lanes : Anti-SNAIL antibody [EPR21043] (ab216347) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
SNAIL expression is not detectable in MCF7 cells, which is consistent with what has been described in the literature (PMID: 10655587 and 22028892).
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![Western blot - Anti-SNAIL antibody [EPR21043] (ab216347)](https://www.abcam.com/ps/products/216/ab216347/Images/ab216347-296141-anti-snail-antibody-epr21043-western-blot.jpg)
Western blot - Anti-SNAIL antibody [EPR21043] (ab216347)All lanes : Anti-SNAIL antibody [EPR21043] (ab216347) at 1/10000 dilution
Lane 1 : His-tagged human SNAIL recombinant protein (aa1-264)
Lane 2 : His-tagged human Slug recombinant protein (aa21-268)
Lane 3 : His-tagged human Slug recombinant protein (aa1-110)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (18)
ab216347 has been referenced in 18 publications.
- Liu Y et al. AFAP1-AS1 induces cisplatin resistance in non-small cell lung cancer through PI3K/AKT pathway. Oncol Lett 19:1024-1030 (2020). WB ; Human . PubMed: 31897216
- Li Y et al. Dihydroartemisinin inhibits the tumorigenesis and metastasis of breast cancer via downregulating CIZ1 expression associated with TGF-ß1 signaling. Life Sci N/A:117454 (2020). WB ; Human . PubMed: 32088211
- Lv X et al. Circ-MMP2 (circ-0039411) induced by FOXM1 promotes the proliferation and migration of lung adenocarcinoma cells in vitro and in vivo. Cell Death Dis 11:426 (2020). WB ; Human . PubMed: 32513952
- Wang B et al. NLRC5 deficiency ameliorates cardiac fibrosis in diabetic cardiomyopathy by regulating EndMT through Smad2/3 signaling pathway. Biochem Biophys Res Commun 528:545-553 (2020). WB ; Human . PubMed: 32505342
- Dong N Long Noncoding RNA NEAT1 Regulates TGF-ß2-Induced Epithelial-Mesenchymal Transition of Lens Epithelial Cells through the miR-34a/Snail1 and miR-204/Zeb1 Pathways. Biomed Res Int 2020:8352579 (2020). WB ; Human . PubMed: 32596382
