Overview

  • Product name

    Anti-SNAIL antibody [EPR21043]
    See all SNAIL primary antibodies
  • Description

    Rabbit monoclonal [EPR21043] to SNAIL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human SNAIL aa 1 to the C-terminus.
    Database link: O95863

  • Positive control

    • WB: His-tagged human SNAIL recombinant protein (aa1-264); HeLa and HCT 116 whole cell lysates. IP: HeLa whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab216347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
IP 1/30.

Target

  • Function

    Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.
  • Tissue specificity

    Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.
  • Sequence similarities

    Belongs to the snail C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • Post-translational
    modifications

    Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.
    Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2.
    O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.
    ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation.
  • Cellular localization

    Nucleus. Cytoplasm. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
  • Information by UniProt
  • Database links

  • Alternative names

    • dJ710H13.1 antibody
    • Protein sna antibody
    • Protein snail homolog 1 antibody
    • Protein snail homolog antibody
    • SLUGH2 antibody
    • SNA antibody
    • Sna protein antibody
    • SNAH antibody
    • SNAI antibody
    • snai1 antibody
    • SNAI1_HUMAN antibody
    • Snail 1 homolog antibody
    • Snail 1 zinc finger protein antibody
    • SNAIL antibody
    • Snail family transcriptional repressor 1 antibody
    • Snail homolog 1 (Drosophila) antibody
    • SNAIL, Drosophila, homolog of, 1 antibody
    • SNAIL1 antibody
    • Zinc finger protein SNAI1 antibody
    see all

Images

  • SNAIL was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab216347 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216347 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (Input).
    Lane 2: ab216347 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216347 in HeLa whole cell lysate.

    Exposure time: 10 seconds.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • All lanes : Anti-SNAIL antibody [EPR21043] (ab216347) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    SNAIL expression is not detectable in MCF7 cells, which is consistent with what has been described in the literature (PMID: 10655587 and 22028892).

  • All lanes : Anti-SNAIL antibody [EPR21043] (ab216347) at 1/10000 dilution

    Lane 1 : His-tagged human SNAIL recombinant protein (aa1-264)
    Lane 2 : His-tagged human Slug recombinant protein (aa21-268)
    Lane 3 : His-tagged human Slug recombinant protein (aa1-110)

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:

  • Zhang YL  et al. High Expression B3GAT3 Is Related with Poor Prognosis of Liver Cancer. Open Med (Wars) 14:251-258 (2019). WB ; Human . Read more (PubMed: 30847403) »
  • Wang XL & Huang C Difference of TGF-ß/Smads signaling pathway in epithelial-mesenchymal transition of normal colonic epithelial cells induced by tumor-associated fibroblasts and colon cancer cells. Mol Biol Rep N/A:N/A (2019). WB ; Human . Read more (PubMed: 30835040) »
See all 5 Publications for this product

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