- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-SNAIL antibody [Sn9H2]
See all SNAIL primary antibodies -
DescriptionRat monoclonal [Sn9H2] to SNAIL
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Host speciesRat
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fusion protein of Human SNAIL with 6x His® tag
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Positive control
- This antibody gave a positive signal when tested against the SNAIL recombinant protein.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
PurityIgG fraction
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ClonalityMonoclonal
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Clone numberSn9H2
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MyelomaP3x63-Ag8.653
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IsotypeIgG2a
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab31787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 10 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
Target
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FunctionInvolved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.
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Tissue specificityExpressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.
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Sequence similaritiesBelongs to the snail C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers. -
Post-translational
modificationsPhosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.
Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2.
O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.
ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation. -
Cellular localizationNucleus. Cytoplasm. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
- Information by UniProt
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Database links
- Entrez Gene: 6615 Human
- Omim: 604238 Human
- SwissProt: O95863 Human
- Unigene: 48029 Human
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Alternative names
- dJ710H13.1 antibody
- Protein sna antibody
- Protein snail homolog 1 antibody
see all
Images
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![Western blot - Anti-SNAIL antibody [Sn9H2] (ab31787)](https://www.abcam.com/ps/products/31/ab31787/Images/SNAIL-Primary-antibodies-ab31787-5.jpg)
Western blot - Anti-SNAIL antibody [Sn9H2] (ab31787)All lanes : Anti-SNAIL antibody [Sn9H2] (ab31787) at 10 µg/ml
Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
Lane 2 : SLUG (SNAI2) Human Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 68 kDa (why is the actual band size different from the predicted?)
Exposure time: 16 minutes
Ab31787 recognizes the tagged recombinant SNAIL protein which has an expected molecular weight of 68 kDa.
Protocols
References
This product has been referenced in:
- Weng W et al. Metastasis-associated protein 1 promotes tumor invasion by downregulation of E-cadherin. Int J Oncol 44:812-8 (2014). Human . Read more (PubMed: 24424621) »
- Fedorova L et al. Down-regulation of the transcription factor snail in the placentas of patients with preeclampsia and in a rat model of preeclampsia. Reprod Biol Endocrinol 10:15 (2012). Read more (PubMed: 22360878) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"