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    snail-antibody-sn9h2-ab31787.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-SNAIL antibody [Sn9H2] (ab31787)

  • Datasheet
Reviews (1)Q&A (1)References (5)

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Western blot - Anti-SNAIL antibody [Sn9H2] (ab31787)

    Key features and details

    • Rat monoclonal [Sn9H2] to SNAIL
    • Suitable for: WB
    • Reacts with: Human, Recombinant fragment
    • Isotype: IgG2a

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    Overview

    • Product name

      Anti-SNAIL antibody [Sn9H2]
      See all SNAIL primary antibodies
    • Description

      Rat monoclonal [Sn9H2] to SNAIL
    • Host species

      Rat
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human, Recombinant fragment
    • Immunogen

      Fusion protein corresponding to Human SNAIL.

    • Positive control

      • This antibody gave a positive signal when tested against the SNAIL recombinant protein.
    • General notes

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 6.97% L-Arginine
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      Sn9H2
    • Myeloma

      P3x63-Ag8.653
    • Isotype

      IgG2a
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Zinc Finger
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Transcription factors
      • Cardiovascular
      • Heart
      • Cardiogenesis
      • Transcription factors/regulators

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Isotype control

      • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
    • Recombinant Protein

      • Recombinant Human SNAIL protein (ab134870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab31787 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 10 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
    Notes
    WB
    Use a concentration of 10 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

    Target

    • Function

      Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.
    • Tissue specificity

      Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.
    • Sequence similarities

      Belongs to the snail C2H2-type zinc-finger protein family.
      Contains 4 C2H2-type zinc fingers.
    • Post-translational
      modifications

      Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.
      Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2.
      O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.
      ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation.
    • Cellular localization

      Nucleus. Cytoplasm. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
    • Target information above from: UniProt accession O95863 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6615 Human
      • Omim: 604238 Human
      • SwissProt: O95863 Human
      • Unigene: 48029 Human
      • Alternative names

        • dJ710H13.1 antibody
        • Protein sna antibody
        • Protein snail homolog 1 antibody
        • Protein snail homolog antibody
        • SLUGH2 antibody
        • SNA antibody
        • Sna protein antibody
        • SNAH antibody
        • SNAI antibody
        • snai1 antibody
        • SNAI1_HUMAN antibody
        • Snail 1 homolog antibody
        • Snail 1 zinc finger protein antibody
        • SNAIL antibody
        • Snail family transcriptional repressor 1 antibody
        • Snail homolog 1 (Drosophila) antibody
        • SNAIL, Drosophila, homolog of, 1 antibody
        • SNAIL1 antibody
        • Zinc finger protein SNAI1 antibody
        see all

      Images

      • Western blot - Anti-SNAIL antibody [Sn9H2] (ab31787)
        Western blot - Anti-SNAIL antibody [Sn9H2] (ab31787)
        All lanes : Anti-SNAIL antibody [Sn9H2] (ab31787) at 10 µg/ml

        Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
        Lane 2 : SLUG (SNAI2) Human Recombinant Protein

        Lysates/proteins at 0.1 µg per lane.

        Secondary
        All lanes : Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution

        Performed under reducing conditions.

        Predicted band size: 29 kDa
        Observed band size: 68 kDa why is the actual band size different from the predicted?


        Exposure time: 16 minutes


        Ab31787 recognizes the tagged recombinant SNAIL protein which has an expected molecular weight of 68 kDa.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (5)

      Publishing research using ab31787? Please let us know so that we can cite the reference in this datasheet.

      ab31787 has been referenced in 5 publications.

      • Tang Z  et al. Enhanced monoacylglycerol lipolysis by ABHD6 promotes NSCLC pathogenesis. EBioMedicine 53:102696 (2020). PubMed: 32143183
      • Wang S  et al. MiR-193a-5p suppresses cell proliferation and induces cell apoptosis by regulating HOXA7 in human ovarian cancer. Neoplasma 67:825-833 (2020). PubMed: 32305054
      • Weng W  et al. Metastasis-associated protein 1 promotes tumor invasion by downregulation of E-cadherin. Int J Oncol 44:812-8 (2014). Human . PubMed: 24424621
      • Fedorova L  et al. Down-regulation of the transcription factor snail in the placentas of patients with preeclampsia and in a rat model of preeclampsia. Reprod Biol Endocrinol 10:15 (2012). PubMed: 22360878
      • Rosivatz E  et al. Expression and nuclear localization of Snail, an E-cadherin repressor, in adenocarcinomas of the upper gastrointestinal tract. Virchows Arch 448:277-87 (2006). PubMed: 16328348

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-2 of 2 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SNAIL antibody [Sn9H2]

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (embrionic mice Lung)
      Specification
      embrionic mice Lung
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris buffer pH 9
      Permeabilization
      No
      Blocking step
      Gelatin 1% / goat serum 10% as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
      Read More

      MR. Raul Peña

      Verified customer

      Submitted Dec 03 2012

      Question

      ReviewID: 33318 AbID: 31787, Anti-SNAIL antibody [Sn9H2] Rating: Poor Image: Anti-SNAIL antibody [Sn9H2] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (Mouse) Sample: Species: Mouse Type: Tissue sections Specification: embrionic mice Lung Application: Application: Fixative: Formaldehyde Antigen retrieval step: Heat mediated - Buffer/Enzyme Used: Tris buffer pH 9 Permeabilization: No Blocking step: Gelatin 1% / goat serum 10% as blocking agent for 2 hours Other detail: Dilution: 1/100 Incubation time: 16 hours at 4°C Diluent: Gelatin 1% / goat serum 2% Secondary Antibody: Name: Non-Abcam Antibody was used: anti Rat IgGs Conjugation: HRP Dilution: 1/100 Additional Data: Additional Notes: No signal using Citrate buffer pH6. No signal in other mice tissues routinarelly used as control (p.ex.: mouse skin or human placenta samples)

      Read More

      Abcam community

      Verified customer

      Asked on Dec 03 2012

      Answer

      Thank you for submitting the review for the Anti-SNAIL antibody [Sn9H2] (ab31787).

      I am sorry you did not find the antibody to produce any staining in your experiment. We have as yet not tested this antibody with mouse samples or in IHC-P, we are therefore not able to guarantee its performance. It may be with further optimisation of the fixation and antigen retrieval process that the antibody may provide better results. It may also be worth considering using a higher concentration of the antibody, 1/10 instead of the current 1/100 being used.

      We do have an alternative antibody against snail which has been used in IHC of mouse samples that you may want to consider. This is the goat polylconal ab53519.

      I hope this information has been of some help. If I can be of any further assistance, please do not hesitate to let me know.

      Read More

      Abcam Scientific Support

      Answered on Dec 03 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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