• Product name
    Anti-SNAIL + SLUG antibody [20C8]
    See all SNAIL + SLUG primary antibodies
  • Description
    Mouse monoclonal [20C8] to SNAIL + SLUG
  • Host species
  • Specificity
    This antibody detects endogonous proteins in the following cell lines: MDAMB-453; MDAMB-435; MDAMB-231; A375.
  • Tested applications
    Suitable for: Sandwich ELISA, ELISA, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)



Our Abpromise guarantee covers the use of ab78105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 1 µg/ml. For sandwich ELISA, use this antibody as Capture at 1 µg/ml with Rabbit polyclonal to SNAIL - ChIP Grade (ab85931) as Detection.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 29 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.


  • Relevance
    Function: SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. PTM: SNAIL is phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Similarity: Both SNAIL and SLUG belong to the snail C2H2-type zinc-finger protein family. Tissue specificity: SNAIL is expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. SLUG is expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
  • Cellular localization
    Slug is generally nuclear, while Snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
  • Database links
  • Alternative names
    • dJ710H13.1 antibody
    • MGC10182 antibody
    • Neural crest transcription factor Slug antibody
    • Protein sna antibody
    • Protein snail homolog 1 antibody
    • Protein snail homolog 2 antibody
    • Protein snail homolog antibody
    • Slug homolog zinc finger protein antibody
    • Slug zinc finger protein antibody
    • SLUGH antibody
    • SLUGH 1 antibody
    • SLUGH1 antibody
    • SLUGH2 antibody
    • SNA antibody
    • Sna protein antibody
    • SNAH antibody
    • SNAI 2 antibody
    • snai1 antibody
    • SNAI1_HUMAN antibody
    • Snai2 antibody
    • SNAI2_HUMAN antibody
    • Snail 2 antibody
    • Snail homolog 1 (Drosophila) antibody
    • Snail homolog 2 antibody
    • Snail2 antibody
    • WS 2D antibody
    • WS2D antibody
    • Zinc finger protein SLUG antibody
    • Zinc finger protein SNAI1 antibody
    • Zinc finger protein SNAI2 antibody
    see all


  • All lanes : Anti-SNAIL + SLUG antibody [20C8] (ab78105) at 1/250 dilution

    Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
    Lane 2 : SLUG (SNAI2) Human Recombinant Protein

    Lysates/proteins at 0.1 µg per lane.

    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 68 kDa
    why is the actual band size different from the predicted?

    Exposure time: 3 minutes

    Ab78105 recognizes the tagged recombinant SLUG and SNAIL proteins which have an expected molecular weight of 68 kDa
  • Standard Curve for SNAIL ; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [20C8] to SNAIL + SLUG (ab78105) at 1µg/ml and Detector Antibody Rabbit polyclonal to SNAIL + SLUG - ChIP Grade (ab85931) at 0.5µg/ml.


This product has been referenced in:
  • Yang HD  et al. T-cell immune regulator 1 enhances metastasis in hepatocellular carcinoma. Exp Mol Med 50:e420 (2018). Read more (PubMed: 29303507) »
  • Goldman O  et al. The mesenchymal transcription factor SNAI-1 instructs human liver specification. Stem Cell Res 17:62-8 (2016). ChIP ; Human . Read more (PubMed: 27240252) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Western blot
Human Cell lysate - whole cell (DAOY, ONS76 Cells)
Loading amount
20 µg
DAOY, ONS76 Cells
Gel Running Conditions
Reduced Denaturing (10% SDS)
Blocking step
Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Dec 10 2009

Human Cell lysate - whole cell (DAOY and ONS76 Cells)
DAOY and ONS76 Cells
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step
Semiquantitative PCR
Positive control
Whole cell lysate
Negative control
Buffer Control

Abcam user community

Verified customer

Submitted Nov 09 2009

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