Key features and details
- Mouse monoclonal [20C8] to SNAIL + SLUG
- Suitable for: Sandwich ELISA, ELISA, WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-SNAIL + SLUG antibody [20C8]
See all SNAIL + SLUG primary antibodies
DescriptionMouse monoclonal [20C8] to SNAIL + SLUG
SpecificityThis antibody detects endogonous proteins in the following cell lines: MDAMB-453; MDAMB-435; MDAMB-231; A375.
Tested applicationsSuitable for: Sandwich ELISA, ELISA, WB, IP, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length protein (Human)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A/G purified
Our Abpromise guarantee covers the use of ab78105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 1 µg/ml. For sandwich ELISA, use this antibody as Capture at 1 µg/ml with Rabbit polyclonal to SNAIL - ChIP Grade (ab85931) as Detection.|
|ELISA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 29 kDa.|
|IP||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent dilution.|
RelevanceFunction: SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. PTM: SNAIL is phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Similarity: Both SNAIL and SLUG belong to the snail C2H2-type zinc-finger protein family. Tissue specificity: SNAIL is expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. SLUG is expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
Cellular localizationSlug is generally nuclear, while Snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
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All lanes : Anti-SNAIL + SLUG antibody [20C8] (ab78105) at 1/250 dilution
Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
Lane 2 : SLUG (SNAI2) Human Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Ab78105 recognizes the tagged recombinant SLUG and SNAIL proteins which have an expected molecular weight of 68 kDa
Standard Curve for SNAIL ; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [20C8] to SNAIL + SLUG (ab78105) at 1µg/ml and Detector Antibody Rabbit polyclonal to SNAIL + SLUG - ChIP Grade (ab85931) at 0.5µg/ml.
ab78105 has been referenced in 3 publications.
- Yang HD et al. T-cell immune regulator 1 enhances metastasis in hepatocellular carcinoma. Exp Mol Med 50:e420 (2018). PubMed: 29303507
- Goldman O et al. The mesenchymal transcription factor SNAI-1 instructs human liver specification. Stem Cell Res 17:62-8 (2016). ChIP ; Human . PubMed: 27240252
- Ju SY et al. Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells. Cell Death Dis 6:e1801 (2015). WB . PubMed: 26136074