Anti-SNAIL + SLUG antibody (ab180714)

Rabbit polyclonal SNAIL + SLUG antibody. Validated in WB, IHC, ICC/IF and tested in Mouse, Rat, Human. Cited in 59 publication(s). Independently reviewed in 6 review(s).

Overview

  • Product name
    Anti-SNAIL + SLUG antibody
    See all SNAIL + SLUG primary antibodies
  • Description
    Rabbit polyclonal to SNAIL + SLUG
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SNAIL+SLUG aa 236-264. The exact sequence is proprietary.
    Database link: O95863

  • Positive control
    • WB: HeLa, A431, MCF7, K563, SW620 and HepG2 cell lysates; mouse lung, mouse heart and rat liver tissue lysates. IHC-P: Human kidney, human colon carcinoma, mouse testis and rat brain tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab180714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
WB 1/500 - 1/2000. Predicted molecular weight: 29 kDa.
ICC/IF 1/50 - 1/200.
IHC-P 1/50 - 1/200.

Target

  • Relevance
    Function: SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. PTM: SNAIL is phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Similarity: Both SNAIL and SLUG belong to the snail C2H2-type zinc-finger protein family. Tissue specificity: SNAIL is expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. SLUG is expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
  • Cellular localization
    Slug is generally nuclear, while Snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
  • Database links
  • Alternative names
    • dJ710H13.1 antibody
    • MGC10182 antibody
    • Neural crest transcription factor Slug antibody
    • Protein sna antibody
    • Protein snail homolog 1 antibody
    • Protein snail homolog 2 antibody
    • Protein snail homolog antibody
    • Slug homolog zinc finger protein antibody
    • Slug zinc finger protein antibody
    • SLUGH antibody
    • SLUGH 1 antibody
    • SLUGH1 antibody
    • SLUGH2 antibody
    • SNA antibody
    • Sna protein antibody
    • SNAH antibody
    • SNAI 2 antibody
    • snai1 antibody
    • SNAI1_HUMAN antibody
    • Snai2 antibody
    • SNAI2_HUMAN antibody
    • Snail 2 antibody
    • Snail homolog 1 (Drosophila) antibody
    • Snail homolog 2 antibody
    • Snail2 antibody
    • WS 2D antibody
    • WS2D antibody
    • Zinc finger protein SLUG antibody
    • Zinc finger protein SNAI1 antibody
    • Zinc finger protein SNAI2 antibody
    see all

Images

  • Anti-SNAIL + SLUG antibody (ab180714) at 1/500 dilution + Mouse heart tissue extracts

    Predicted band size: 29 kDa

  • All lanes : Anti-SNAIL + SLUG antibody (ab180714)

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Mouse lung tissue lysate
    Lane 5 : Rat liver tissue lysate

    Predicted band size: 29 kDa

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling SNAI1+SLUG with ab180714 at 1/50 dilution.

  • ab180714 staining SNAIL in human MCF10A cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 3.7% PFA in PBS for 15 minutes and blocked with 0.1% Milk + 0.1% Tween in PBS for 30 minutes at 25°C. Samples were incubated with primary antibody (1/1000 in PBS + 0.1% Tween) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling SNAIL + SLUG with ab180714 at 1/100 dilution.

  • Western blot analysis of extracts of various cell lines using ab180714 at the dilution 1/500.

  • ab180714 staining SNAIL in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation in a basic buffer (TRIS+EDTA, pH9). Samples were incubated with primary antibody (1/500 in antibody diluent) for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification: 200X.

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling SNAIL +SLUG with ab180714 at 1/100 dilution.

  • Lane 1: Snail recombinant 0.1 ug (55 kDa)

    Lane 2: Slug recombinant 0.1 ug  (56 kDa)

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification: 400X.

  • ab180714 staining SNAIL in mouse embryonic heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100 in blocking buffer) for 16 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Dai J  et al. Matrix stiffness regulates epithelial-mesenchymal transition via cytoskeletal remodeling and MRTF-A translocation in osteosarcoma cells. J Mech Behav Biomed Mater 90:226-238 (2019). Read more (PubMed: 30384218) »
  • Chen S  et al. RER1 enhances carcinogenesis and stemness of pancreatic cancer under hypoxic environment. J Exp Clin Cancer Res 38:15 (2019). Read more (PubMed: 30630537) »
See all 63 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-6 of 6 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Basic buffer (TRIS+EDTA, pH9)
Permeabilization
No
Specification
Kidney
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 12 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% TritonX100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 07 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 25°C
Sample
Human Cell (MCF10A)
Specification
MCF10A
Permeabilization
No
Fixative
3,7% PFA in PBS, 15mins

Abcam user community

Verified customer

Submitted Sep 29 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Cell lysate - whole cell (MCF10A)
Specification
MCF10A
Treatment
TGFb2 2ng/ml for 48h
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 26 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Mouse Cell lysate - whole cell (mouse pre-iPS neural cells)
Specification
mouse pre-iPS neural cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Aug 21 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Mouse Tissue sections (embryonic heart)
Specification
embryonic heart
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 03 2014

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