Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-SNAIL + SLUG (phospho S246) antibody
See all SNAIL + SLUG primary antibodies -
DescriptionRabbit polyclonal to SNAIL + SLUG (phospho S246)
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Host speciesRabbit
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Specificityab63568 detects endogenous levels of SNAIL only when phosphorylated at serine 246.
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Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthesized phosphopeptide derived from human SNAIL around the phosphorylation site of serine 246 (T-F-SP-R-M).
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Positive control
- H29 nuclear lysate HuvEc cells
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesAffinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab63568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa). | |
| ICC/IF | 1/500 - 1/1000. | |
| ELISA | 1/5000. |
Target
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RelevanceFunction: SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. PTM: SNAIL is phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Similarity: Both SNAIL and SLUG belong to the snail C2H2-type zinc-finger protein family. Tissue specificity: SNAIL is expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. SLUG is expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
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Cellular localizationSlug is generally nuclear, while Snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
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Database links
- Entrez Gene: 6591 Human
- Entrez Gene: 6615 Human
- Entrez Gene: 20583 Mouse
- Entrez Gene: 20613 Mouse
- Omim: 604238 Human
- SwissProt: O43623 Human
- SwissProt: O95863 Human
- SwissProt: P97469 Mouse
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Alternative names
- dJ710H13.1 antibody
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
see all
Images
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Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)All lanes : Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) at 1/500 dilution
Lane 1 : Extracts from HT29 cells (5-30µg), minus immunising peptide
Lane 2 : Extracts from HT29 cells (5-30µg) plus immunising peptide (5-10µg)
Predicted band size: 29 kDa
Observed band size: 26 kDa (why is the actual band size different from the predicted?) -
Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)All lanes : Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) at 1/500 dilution
Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
Lane 2 : SLUG (SNAI2) Human Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 68 kDa (why is the actual band size different from the predicted?)
Exposure time: 3 minutes
Ab63568 recognizes the tagged recombinant SLUG and SNAIL proteins which have an expected molecular weight of 68 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)Immunofluorescent staining of HuvEc cells, using ab63568 at a 1/500 dilution, minus immunising peptide (left image) and plus immunising peptide (right image).
References
This product has been referenced in:
- Tong R et al. KCTD11 inhibits growth and metastasis of hepatocellular carcinoma through activating Hippo signaling. Oncotarget 8:37717-37729 (2017). Read more (PubMed: 28465479) »
- Magnusson C et al. Regulation of cysteinyl leukotriene receptor 2 expression-a potential anti-tumor mechanism. PLoS One 6:e29060 (2011). WB ; Human . Read more (PubMed: 22194989) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"