Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)

Reviews (1)Q&A (3)References (4)

Overview

  • Product name

    Anti-SNAIL + SLUG (phospho S246) antibody
    See all SNAIL + SLUG primary antibodies

  • Description

    Rabbit polyclonal to SNAIL + SLUG (phospho S246)

  • Host species

    Rabbit

  • Specificity

    ab63568 detects endogenous levels of SNAIL only when phosphorylated at serine 246.

  • Tested applications

    Suitable for: WB, ICC/IF, ELISAmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthesized phosphopeptide derived from human SNAIL around the phosphorylation site of serine 246 (T-F-SP-R-M).

  • Positive control

    • H29 nuclear lysate HuvEc cells

Properties

Applications

Our Abpromise guarantee covers the use of ab63568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

Target

  • Relevance

    Function: SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. PTM: SNAIL is phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Similarity: Both SNAIL and SLUG belong to the snail C2H2-type zinc-finger protein family. Tissue specificity: SNAIL is expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. SLUG is expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.

  • Cellular localization

    Slug is generally nuclear, while Snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.

  • Database links

    see all

  • Alternative names

    • dJ710H13.1 antibody
    • MGC10182 antibody
    • Neural crest transcription factor Slug antibody
    • Protein sna antibody
    • Protein snail homolog 1 antibody
    • Protein snail homolog 2 antibody
    • Protein snail homolog antibody
    • Slug homolog zinc finger protein antibody
    • Slug zinc finger protein antibody
    • SLUGH antibody
    • SLUGH 1 antibody
    • SLUGH1 antibody
    • SLUGH2 antibody
    • SNA antibody
    • Sna protein antibody
    • SNAH antibody
    • SNAI 2 antibody
    • snai1 antibody
    • SNAI1_HUMAN antibody
    • Snai2 antibody
    • SNAI2_HUMAN antibody
    • Snail 2 antibody
    • Snail homolog 1 (Drosophila) antibody
    • Snail homolog 2 antibody
    • Snail2 antibody
    • WS 2D antibody
    • WS2D antibody
    • Zinc finger protein SLUG antibody
    • Zinc finger protein SNAI1 antibody
    • Zinc finger protein SNAI2 antibody
    see all

Images

  • Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)
    Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)
    All lanes : Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) at 1/500 dilution

    Lane 1 : Extracts from HT29 cells (5-30µg), minus immunising peptide
    Lane 2 : Extracts from HT29 cells (5-30µg) plus immunising peptide (5-10µg)

    Predicted band size: 29 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)
    Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)
    All lanes : Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) at 1/500 dilution

    Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
    Lane 2 : SLUG (SNAI2) Human Recombinant Protein

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 68 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Ab63568 recognizes the tagged recombinant SLUG and SNAIL proteins which have an expected molecular weight of 68 kDa
  • Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)
    Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)
    Immunofluorescent staining of HuvEc cells, using ab63568 at a 1/500 dilution, minus immunising peptide (left image) and plus immunising peptide (right image).

References

This product has been referenced in:

  • Chow HY  et al. Group I Paks are essential for epithelial- mesenchymal transition in an Apc-driven model of colorectal cancer. Nat Commun 9:3473 (2018). Read more (PubMed: 30150766) »
  • Yan H  et al. Long noncoding RNA MLK7-AS1 promotes ovarian cancer cells progression by modulating miR-375/YAP1 axis. J Exp Clin Cancer Res 37:237 (2018). Read more (PubMed: 30249278) »
See all 4 Publications for this product

Publishing research using ab63568? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SNAIL + SLUG (phospho S246) antibody

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tongue)
Specification
tongue
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Tris pH=9.0 / 1 mM EDTA
Permeabilization
No
Read More

Abcam user community

Verified customer

Submitted Jan 29 2013

Question

BTW, the secondary antibody I used for the anti-snail primary was goat anti-rabbit-HRP not goat anti-Snail. Sorry for the mistake.

Read More
Answer

Thank you for your email. I apologize for the delay as I have to leave for a conference.

I have also received an answer from lab. They have suggested that the recombinant protein they have used is tagged with GST which is why the band size seen in image is 68 kDa rather than 29-30 kDa.

Looking at the provided image and details, the antibody ab53519 seems detecting the correct target however the protocol might need optimizations in case of ab63568.

I would suggest following updates for protocol
- use 5% BSA for antibody dilutions and blocking and block membrane for 1-2 hours at RT.
- Try no primary control
- Heat lysates with sample buffer for 10 minutes at 100C.

I am sure these tips will be helpful. Should you observe similar results please do not hesitate to contact me.

Read More

Question

Dear whom it may concern,
Hello. I recently purchased two antibodies from your company, anti-SNAIL and anti-SNAIL+SLUG (phospho S246) to see the phosphorylated form of SNAIL. Since one is detecting SNAIL and the other is detecting phosphorylated form of SNAIL, I was expecting to see bands of similar sizes if not the same, but your data sheets were saying 68 kDa for SNAIL and 26 kDa for phospho-SNAIL. And when I tried the antibodies myself to do Western blotting, my results were not very different: my SNAIL bands did not match with my phospho-SNAIL bands, in fact, I was confused which bands were my phosphorylated SNAIL bands.Why are the sizes markedly different? Please reply to

Read More
Answer

Thank you for contacting us.

Could you provide the following details

- Cell type e.g. Human hela cells lysates
- Provide image
- Blocking conditions for both antibodies
- Dilutions of both primary antibodies
- Information about secondary antibody
- How long the antibodies were incubated?

we will then look into details why the observed band size is different than predicted.

Read More

Question

The refund has hit the credit card.  Is there a way you or have someone, send me a copy of the credit memo for this?   Thanks so much,  

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Answer

Thanks for contacting us. I have asked our accounting department to email a copy of the credit note to you. If you need anything else, feel free to contact me or send an email to our accounting department at us.credits@abcam.com.

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