Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)


  • Product name
    Anti-SNAIL + SLUG (phospho S246) antibody
    See all SNAIL + SLUG primary antibodies
  • Description
    Rabbit polyclonal to SNAIL + SLUG (phospho S246)
  • Host species
  • Specificity
    ab63568 detects endogenous levels of SNAIL only when phosphorylated at serine 246.
  • Tested applications
    Suitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized phosphopeptide derived from human SNAIL around the phosphorylation site of serine 246 (T-F-SP-R-M).

  • Positive control
    • H29 nuclear lysate HuvEc cells



Our Abpromise guarantee covers the use of ab63568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.


  • Relevance
    Function: SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. PTM: SNAIL is phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Similarity: Both SNAIL and SLUG belong to the snail C2H2-type zinc-finger protein family. Tissue specificity: SNAIL is expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. SLUG is expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
  • Cellular localization
    Slug is generally nuclear, while Snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
  • Database links
  • Alternative names
    • dJ710H13.1 antibody
    • MGC10182 antibody
    • Neural crest transcription factor Slug antibody
    • Protein sna antibody
    • Protein snail homolog 1 antibody
    • Protein snail homolog 2 antibody
    • Protein snail homolog antibody
    • Slug homolog zinc finger protein antibody
    • Slug zinc finger protein antibody
    • SLUGH antibody
    • SLUGH 1 antibody
    • SLUGH1 antibody
    • SLUGH2 antibody
    • SNA antibody
    • Sna protein antibody
    • SNAH antibody
    • SNAI 2 antibody
    • snai1 antibody
    • SNAI1_HUMAN antibody
    • Snai2 antibody
    • SNAI2_HUMAN antibody
    • Snail 2 antibody
    • Snail homolog 1 (Drosophila) antibody
    • Snail homolog 2 antibody
    • Snail2 antibody
    • WS 2D antibody
    • WS2D antibody
    • Zinc finger protein SLUG antibody
    • Zinc finger protein SNAI1 antibody
    • Zinc finger protein SNAI2 antibody
    see all


  • All lanes : Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) at 1/500 dilution

    Lane 1 : Extracts from HT29 cells (5-30µg), minus immunising peptide
    Lane 2 : Extracts from HT29 cells (5-30µg) plus immunising peptide (5-10µg)

    Predicted band size: 29 kDa
    Observed band size: 26 kDa (why is the actual band size different from the predicted?)

  • All lanes : Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) at 1/500 dilution

    Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
    Lane 2 : SLUG (SNAI2) Human Recombinant Protein

    Lysates/proteins at 0.1 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 68 kDa (why is the actual band size different from the predicted?)

    Exposure time: 3 minutes

    Ab63568 recognizes the tagged recombinant SLUG and SNAIL proteins which have an expected molecular weight of 68 kDa
  • Immunofluorescent staining of HuvEc cells, using ab63568 at a 1/500 dilution, minus immunising peptide (left image) and plus immunising peptide (right image).


This product has been referenced in:
  • Tong R  et al. KCTD11 inhibits growth and metastasis of hepatocellular carcinoma through activating Hippo signaling. Oncotarget 8:37717-37729 (2017). Read more (PubMed: 28465479) »
  • Magnusson C  et al. Regulation of cysteinyl leukotriene receptor 2 expression-a potential anti-tumor mechanism. PLoS One 6:e29060 (2011). WB ; Human . Read more (PubMed: 22194989) »

See all 2 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (tongue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Tris pH=9.0 / 1 mM EDTA

Abcam user community

Verified customer

Submitted Jan 29 2013

Thank you for your email. I apologize for the delay as I have to leave for a conference.

I have also received an answer from lab. They have suggested that the recombinant protein they have used is tagged with GST which is why the band size see...

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Thank you for contacting us.

Could you provide the following details

- Cell type e.g. Human hela cells lysates
- Provide image
- Blocking conditions for both antibodies
- Dilutions of both primary antibodies
- Informat...

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Thanks for contacting us. I have asked our accounting department to email a copy of the credit note to you. If you need anything else, feel free to contact me or send an email to our accounting department at


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