Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)
Key features and details
- Rabbit polyclonal to SNAIL + SLUG (phospho S246)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SNAIL + SLUG (phospho S246) antibody
See all SNAIL + SLUG primary antibodies -
Description
Rabbit polyclonal to SNAIL + SLUG (phospho S246) -
Host species
Rabbit -
Specificity
ab63568 detects endogenous levels of SNAIL only when phosphorylated at serine 246. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthesized phosphopeptide derived from human SNAIL around the phosphorylation site of serine 246 (T-F-SP-R-M).
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Positive control
- H29 nuclear lysate HuvEc cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab63568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa). | |
ICC/IF | 1/500 - 1/1000. |
Target
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Relevance
Function: SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. PTM: SNAIL is phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Similarity: Both SNAIL and SLUG belong to the snail C2H2-type zinc-finger protein family. Tissue specificity: SNAIL is expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. SLUG is expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. -
Cellular localization
Slug is generally nuclear, while Snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs. -
Database links
- Entrez Gene: 6591 Human
- Entrez Gene: 6615 Human
- Entrez Gene: 20583 Mouse
- Entrez Gene: 20613 Mouse
- Omim: 604238 Human
- SwissProt: O43623 Human
- SwissProt: O95863 Human
- SwissProt: P97469 Mouse
see all -
Alternative names
- dJ710H13.1 antibody
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
see all
Images
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All lanes : Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) at 1/500 dilution
Lane 1 : Extracts from HT29 cells (5-30µg), minus immunising peptide
Lane 2 : Extracts from HT29 cells (5-30µg) plus immunising peptide (5-10µg)
Predicted band size: 29 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted? -
All lanes : Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) at 1/500 dilution
Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
Lane 2 : SLUG (SNAI2) Human Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Ab63568 recognizes the tagged recombinant SLUG and SNAIL proteins which have an expected molecular weight of 68 kDa -
Immunofluorescent staining of HuvEc cells, using ab63568 at a 1/500 dilution, minus immunising peptide (left image) and plus immunising peptide (right image).
Protocols
Datasheets and documents
References (4)
ab63568 has been referenced in 4 publications.
- Chow HY et al. Group I Paks are essential for epithelial- mesenchymal transition in an Apc-driven model of colorectal cancer. Nat Commun 9:3473 (2018). PubMed: 30150766
- Yan H et al. Long noncoding RNA MLK7-AS1 promotes ovarian cancer cells progression by modulating miR-375/YAP1 axis. J Exp Clin Cancer Res 37:237 (2018). PubMed: 30249278
- Tong R et al. KCTD11 inhibits growth and metastasis of hepatocellular carcinoma through activating Hippo signaling. Oncotarget 8:37717-37729 (2017). PubMed: 28465479
- Magnusson C et al. Regulation of cysteinyl leukotriene receptor 2 expression-a potential anti-tumor mechanism. PLoS One 6:e29060 (2011). WB ; Human . PubMed: 22194989