Recombinant Anti-SNAP-beta antibody [EPR15046(B)] - BSA and Azide free (ab250764)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15046(B)] to SNAP-beta - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SNAP-beta antibody [EPR15046(B)] - BSA and Azide free
See all SNAP-beta primary antibodies -
Description
Rabbit monoclonal [EPR15046(B)] to SNAP-beta - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250764 is the carrier-free version of ab184563.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15046(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
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Notes |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
Target
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Relevance
Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus. -
Cellular localization
Membrane; Peripheral membrane protein -
Database links
- Entrez Gene: 63908 Human
- Entrez Gene: 17957 Mouse
- Entrez Gene: 499903 Rat
- Omim: 611270 Human
- SwissProt: Q9H115 Human
- SwissProt: P28663 Mouse
- SwissProt: P85969 Rat
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Alternative names
- Beta soluble NSF attachment protein antibody
- N ethylmaleimide sensitive factor attachment protein beta antibody
- NAPB antibody
see all
Images
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All lanes : Anti-SNAP-beta antibody [EPR15046(B)] - N-terminal (ab184563) at 1/5000 dilution
Lane 1 : A549 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 34 kDaThis data was developed using ab184563, the same antibody clone in a different buffer formulation.
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This data was developed using ab184563, the same antibody clone in a different buffer formulation.
Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab184563 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
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Anti-SNAP-beta antibody [EPR15046(B)] - N-terminal (ab184563) at 1/5000 dilution + Human fetal brain lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 34 kDaThis data was developed using ab184563, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250764 has not yet been referenced specifically in any publications.