Anti-SNAP23 antibody (ab166808)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-SNAP23 antibody
See all SNAP23 primary antibodies -
DescriptionGoat polyclonal to SNAP23
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Host speciesGoat
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Specificityab166808 is expected to recognize both reported isoforms (NP_003816.2; NP_570710.1).
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Cow, Human
Predicted to work with: Monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide:
QIKRITDKAD
by a Cysteine residue linker, corresponding to internal sequence amino acids 183-192 of Human SNAP23 (NP_003816.2). -
Positive control
- Human placenta lysate; Human spleen tissue
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab166808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa). | |
| IHC-P | Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionEssential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.
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Tissue specificityUbiquitous. Highest levels where found in placenta.
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Sequence similaritiesBelongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains. -
Cellular localizationCell membrane. Cell membrane. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane.
- Information by UniProt
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Database links
- Entrez Gene: 522423 Cow
- Entrez Gene: 101141000 Gorilla
- Entrez Gene: 8773 Human
- Entrez Gene: 20619 Mouse
- Entrez Gene: 100174679 Orangutan
- Entrez Gene: 64630 Rat
- Omim: 602534 Human
- SwissProt: O00161 Human
see all -
Alternative names
- HsT17016 antibody
- LS-B8340 antibody
- SNAP 23 antibody
see all
Images
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Western blot - Anti-SNAP23 antibody (ab166808)Anti-SNAP23 antibody (ab166808) at 0.1 µg/ml + Human Placenta lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 23 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (ab166808)Immunohistochemical analysis of formalin fixed, paraffin embedded Human spleen tissue labeling SNAP23 with ab166808 antibody at 3.75 µg/ml.
Datasheets and documents
References
This product has been referenced in:
- da Silva LB et al. Botulinum neurotoxin type A modulates vesicular release of glutamate from satellite glial cells. J Cell Mol Med 19:1900-9 (2015). ICC, IHC . Read more (PubMed: 25754332) »
