Overview

  • Product name
  • Description
    Rabbit polyclonal to SNAP23
  • Host species
    Rabbit
  • Specificity
    This antibody reacts specifically with human SNAP23.
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein corresponding to Human SNAP23. Immunogen is a SNAP23-GST fusion protein
    Database link: O00161

Properties

Applications

Our Abpromise guarantee covers the use of ab4114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50 - 1/200. PubMed: 17272274
WB 1/250 - 1/1000.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50.
IP Use at an assay dependent concentration. PubMed: 22393054

Target

  • Function
    Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.
  • Tissue specificity
    Ubiquitous. Highest levels where found in placenta.
  • Sequence similarities
    Belongs to the SNAP-25 family.
    Contains 2 t-SNARE coiled-coil homology domains.
  • Cellular localization
    Cell membrane. Cell membrane. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • HsT17016 antibody
    • LS-B8340 antibody
    • SNAP 23 antibody
    • SNAP-23 antibody
    • SNAP23 antibody
    • SNAP23A antibody
    • SNAP23B antibody
    • SNP23_HUMAN antibody
    • Synaptosomal associated protein 23 antibody
    • Synaptosomal associated protein 23kDa antibody
    • Synaptosomal associated protein antibody
    • Synaptosomal-associated protein 23 antibody
    • Vesicle membrane fusion protein SNAP 23 antibody
    • Vesicle membrane fusion protein SNAP23 antibody
    • Vesicle-membrane fusion protein SNAP-23 antibody
    see all

Images

  • Taken from Galli et al. 1998. Taken from Galli et al. 1998.
  • IHC image of ab4114 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4114, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab4114 staining SNAP23 in HT-1080 cells by Immunocytochemistry/ Immunofluorescence.
    Cells were grown on glass coverslips, treated with PMA and then fixed with 4% (w/v) paraformaldehyde in phosphate buffered saline (PBS). Samples were then permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% (w/v) skimmed milk powder in PBS before staining with ab4114 and the appropriate secondary antibody, followed by washing and mounting.

    Scale bars: 10 µm.
  • Immunohistochemical analysis of rat pancreatic lobules, staining SNAP23 with ab4114. Following fixation in paraformaldehyde, cells were blocked for 30 min at RT, and primary antibody (1/50) was added simultaneously for 1.5 hours at RT. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.

References

This product has been referenced in:
  • Bertuccio CA  et al. Plasma membrane insertion of KCa2.3 (SK3) is dependent upon the SNARE proteins, syntaxin-4 and SNAP23. PLoS One 13:e0196717 (2018). Read more (PubMed: 29768434) »
  • Lesteberg K  et al. Recycling endosomes in human cytotoxic T lymphocytes constitute an auxiliary intracellular trafficking pathway for newly synthesized perforin. Immunol Res 65:1031-1045 (2017). Flow Cyt ; Human . Read more (PubMed: 28822075) »
See all 14 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Lung)
Loading amount
20 µg
Specification
Lung
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%

Mrs. Katelyn Miller

Verified customer

Submitted Jun 25 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (neurospheres)
Specification
neurospheres
Fixative
Paraformaldehyde
Permeabilization
Yes - triton-x
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Sep 05 2007

Answer

I'm sorry to inform you have we have no data for the cross reactivity of this anitbody with mouse or other rodent synaptosome associated protein 23. I am not familiar with the degree of sequence homology between human and murine SNAP23. However, as the antibody is a polyclonal it will recognise multiple epitopes across SNAP23 and is therefore more likely to cross react with the protein from different species. If you are still interested in this antibody, please contact me directly. Danielle N. Miller PhD Head of Customer Services danielle.miller@abcam.com

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Question
Answer

No, sorry. The only control is with the endogenous protein from the cell line described in the data sheet.

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Answer

This antibody has not yet been tested for cross-reactivity against rat or mouse SNAP-23.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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