Product nameAnti-SNAP23 antibody
See all SNAP23 primary antibodies
DescriptionRabbit polyclonal to SNAP23
SpecificityThis antibody reacts specifically with human SNAP23.
Tested applicationsSuitable for: IHC-Fr, WB, IHC-P, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Fusion protein corresponding to Human SNAP23. Immunogen is a SNAP23-GST fusion protein
Database link: O00161
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: Whole serum
Our Abpromise guarantee covers the use of ab4114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/50 - 1/200. PubMed: 17272274|
|WB||1/250 - 1/1000.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at an assay dependent concentration. PubMed: 22393054|
FunctionEssential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.
Tissue specificityUbiquitous. Highest levels where found in placenta.
Sequence similaritiesBelongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains.
Cellular localizationCell membrane. Cell membrane. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane.
- Information by UniProt
- HsT17016 antibody
- LS-B8340 antibody
- SNAP 23 antibody
Taken from Galli et al. 1998. Taken from Galli et al. 1998.
IHC image of ab4114 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4114, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab4114 staining SNAP23 in HT-1080 cells by Immunocytochemistry/ Immunofluorescence.
Cells were grown on glass coverslips, treated with PMA and then fixed with 4% (w/v) paraformaldehyde in phosphate buffered saline (PBS). Samples were then permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% (w/v) skimmed milk powder in PBS before staining with ab4114 and the appropriate secondary antibody, followed by washing and mounting.
Scale bars: 10 µm.
Immunohistochemical analysis of rat pancreatic lobules, staining SNAP23 with ab4114. Following fixation in paraformaldehyde, cells were blocked for 30 min at RT, and primary antibody (1/50) was added simultaneously for 1.5 hours at RT. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.
This product has been referenced in:
- Bertuccio CA et al. Plasma membrane insertion of KCa2.3 (SK3) is dependent upon the SNARE proteins, syntaxin-4 and SNAP23. PLoS One 13:e0196717 (2018). Read more (PubMed: 29768434) »
- Lesteberg K et al. Recycling endosomes in human cytotoxic T lymphocytes constitute an auxiliary intracellular trafficking pathway for newly synthesized perforin. Immunol Res 65:1031-1045 (2017). Flow Cyt ; Human . Read more (PubMed: 28822075) »