Product nameAnti-SNAP23 antibody [EPR8538]
See all SNAP23 primary antibodies
DescriptionRabbit monoclonal [EPR8538] to SNAP23
Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human SNAP23 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: O00161
- HeLa, Human placenta, BxPC-3, HepG2 and JAR cell lysates; Human colon tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.45 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 23 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
For unpurified use at 1/50 - 1/100.
FunctionEssential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.
Tissue specificityUbiquitous. Highest levels where found in placenta.
Sequence similaritiesBelongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains.
Cellular localizationCell membrane. Cell membrane. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane.
- Information by UniProt
- HsT17016 antibody
- LS-B8340 antibody
- SNAP 23 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SNAP23 knockout HAP1 cell lysate (20 µg)
Lane 3: HEK293 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab131242 observed at 25 kDa. Red - loading control, ab8245, observed at 37kDa.
ab131242 was shown to recognize SNAP23 when SNAP23 knockout samples were used, along with additional cross-reactive bands. Wild-type and SNAP23 knockout samples were subjected to SDS-PAGE. ab131242 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling SNAP23 with ab131242 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling SNAP23 with purified ab131242 at 1/500 dilution. Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SNAP23 with unpurified ab131242 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
All lanes : Anti-SNAP23 antibody [EPR8538] (ab131242) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human placenta cell lysate
Lane 3 : BxPC-3 cell lysate
Lane 4 : HepG2 cell lysat
Lane 5 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 23 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Zhu B et al. SNAP23 suppresses cervical cancer progression via modulating the cell cycle. Gene 673:217-224 (2018). Read more (PubMed: 29908998) »
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). Read more (PubMed: 30377371) »