Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)

Submit a review Submit a question
Flow Cytometry - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)
  • Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)
  • OI-RD Scanning - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)
  • Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8538] to SNAP23 - Low endotoxin, Azide free
  • Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free
    See all SNAP23 primary antibodies

  • Description

    Rabbit monoclonal [EPR8538] to SNAP23 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human SNAP23 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O00161

  • Positive control

    • HeLa, Human placenta, BxPC-3, HepG2 and JAR cell lysates; Human colon tissue

  • General notes

    ab222224 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab222224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.

    • Tissue specificity

      Ubiquitous. Highest levels where found in placenta.

    • Sequence similarities

      Belongs to the SNAP-25 family.
      Contains 2 t-SNARE coiled-coil homology domains.

    • Cellular localization

      Cell membrane. Cell membrane. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • HsT17016 antibody
        • LS-B8340 antibody
        • SNAP 23 antibody
        • SNAP-23 antibody
        • SNAP23 antibody
        • SNAP23A antibody
        • SNAP23B antibody
        • SNP23_HUMAN antibody
        • Synaptosomal associated protein 23 antibody
        • Synaptosomal associated protein 23kDa antibody
        • Synaptosomal associated protein antibody
        • Synaptosomal-associated protein 23 antibody
        • Vesicle membrane fusion protein SNAP 23 antibody
        • Vesicle membrane fusion protein SNAP23 antibody
        • Vesicle-membrane fusion protein SNAP-23 antibody
        see all

      Images

      • Flow Cytometry - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)
        Flow Cytometry - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)

        Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SNAP23 with unpurified ab131242 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131242).

      • Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)
        Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)

        Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling SNAP23 with purified ab131242 at 1/500 dilution. Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131242).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)

        Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling SNAP23 with ab131242 at 1/100 dilution.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131242).

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • OI-RD Scanning - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)
        OI-RD Scanning - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131242).

      • Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)
        Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)

      References (0)

      Publishing research using ab222224? Please let us know so that we can cite the reference in this datasheet.

      ab222224 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab222224.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com