Recombinant Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free
See all SNAP23 primary antibodies -
Description
Rabbit monoclonal [EPR8538] to SNAP23 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human SNAP23 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: O00161 -
Positive control
- HeLa, Human placenta, BxPC-3, HepG2 and JAR cell lysates; Human colon tissue
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General notes
ab222224 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 2.45 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8538 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab222224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 23 kDa. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion. -
Tissue specificity
Ubiquitous. Highest levels where found in placenta. -
Sequence similarities
Belongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains. -
Cellular localization
Cell membrane. Cell membrane. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 8773 Human
- Omim: 602534 Human
- SwissProt: O00161 Human
- Unigene: 511149 Human
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Alternative names
- HsT17016 antibody
- LS-B8340 antibody
- SNAP 23 antibody
see all
Images
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![Flow Cytometry - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)](https://www.abcam.com/ps/products/222/ab222224/Images/ab222224-4-ab131242275841ab131242FC1.jpg)
Flow Cytometry - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SNAP23 with unpurified ab131242 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131242).
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![Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)](https://www.abcam.com/ps/products/222/ab222224/Images/ab222224-3-ab1312425ab131242Helamethanol500.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling SNAP23 with purified ab131242 at 1/500 dilution. Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131242).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)](https://www.abcam.com/ps/products/222/ab222224/Images/ab222224-2-SNAP23Primaryantibodiesab1312423.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling SNAP23 with ab131242 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131242).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Other - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)](https://www.abcam.com/ps/products/222/ab222224/Images/ab222224-1-SNAP23Primaryantibodiesab1312424.jpg)
Other - Anti-SNAP23 antibody [EPR8538] - Low endotoxin, Azide free (ab222224)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131242).
Protocols
Datasheets and documents
References
ab222224 has not yet been referenced specifically in any publications.
