Anti-SNAP25 antibody (ab228880)
Key features and details
- Rabbit polyclonal to SNAP25
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SNAP25 antibody
See all SNAP25 primary antibodies -
Description
Rabbit polyclonal to SNAP25 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Xenopus laevis, Chimpanzee, Rhesus monkey -
Immunogen
Recombinant fragment within Human SNAP25 (internal sequence). The exact sequence is proprietary.
Database link: P60880 -
Positive control
- WB: Rat brain lysate; Mouse brain lysate; HeLa whole cell lysate. IHC-P: Mouse fore brain tiisue; Rat hind brain tissue; SW480 xenograft tissue. IHC-Fr: E13.5 rat brain tissue. ICC/IF: DIV9 rat E18 primary cortical neurons.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 23 kDa.
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IHC-P |
1/100 - 1/1000.
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IHC-Fr |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 23 kDa. |
IHC-P
1/100 - 1/1000. |
IHC-Fr
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. -
Tissue specificity
Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. -
Sequence similarities
Belongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains. -
Post-translational
modificationsPalmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association. -
Cellular localization
Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 396444 Chicken
- Entrez Gene: 458096 Chimpanzee
- Entrez Gene: 540853 Cow
- Entrez Gene: 6616 Human
- Entrez Gene: 20614 Mouse
- Entrez Gene: 25012 Rat
- Entrez Gene: 574204 Rhesus monkey
- Omim: 600322 Human
see all -
Alternative names
- bA416N4.2 antibody
- Bdr antibody
- CMS18 antibody
see all
Images
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Anti-SNAP25 antibody (ab228880) at 1/1000 dilution + Rat brain lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 23 kDa12% SDS-PAGE gel.
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Anti-SNAP25 antibody (ab228880) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 23 kDa12% SDS-PAGE gel.
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Anti-SNAP25 antibody (ab228880) at 1/1000 dilution + Mouse brain lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 23 kDa12% SDS-PAGE gel.
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Paraffin-embedded mouse fore brain tissue stained for SNAP25 using ab228880 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded rat hind brain tissue stained for SNAP25 using ab228880 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded SW480 (human) xenograft tissue stained for SNAP25 using ab228880 at 1/100 dilution in immunohistochemical analysis.
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Frozen sectioned E13.5 rat brain tissue stained for SNAP25 using ab228880 at 1/250 dilution in immunohistochemical analysis (green).
The nuclear counterstain is DAPI (blue). Beta Tubulin 3, a mature neuron marker, is detected with a beta Tubulin 3 antibody at 1/500 dilution (red).
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4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons cells stained for SNAP25 (green) using ab228880 at 1/500 dilution in ICC/IF.
Nuclear counterstain: DAPI (blue). Beta Tubulin 3, a mature neuron marker, is detected with a beta Tubulin 3 antibody at 1/500 dilution (red).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228880 has not yet been referenced specifically in any publications.