Overview

  • Product name
  • Description
    Rabbit polyclonal to SNAP25
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Xenopus laevis, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human SNAP25 (internal sequence). The exact sequence is proprietary.
    Database link: P60880

  • Positive control
    • WB: Rat brain lysate; Mouse brain lysate; HeLa whole cell lysate. IHC-P: Mouse fore brain tiisue; Rat hind brain tissue; SW480 xenograft tissue. IHC-Fr: E13.5 rat brain tissue. ICC/IF: DIV9 rat E18 primary cortical neurons.

Properties

Applications

Our Abpromise guarantee covers the use of ab228880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 23 kDa.
IHC-P 1/100 - 1/1000.
IHC-Fr 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
  • Tissue specificity
    Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
  • Sequence similarities
    Belongs to the SNAP-25 family.
    Contains 2 t-SNARE coiled-coil homology domains.
  • Post-translational
    modifications
    Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
  • Cellular localization
    Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA416N4.2 antibody
    • Bdr antibody
    • CMS18 antibody
    • dJ1068F16.2 antibody
    • FLJ23079 antibody
    • HGNC:11132 antibody
    • MGC105414 antibody
    • MGC139754 antibody
    • Resistance to inhibitors of cholinesterase 4 homolog antibody
    • RIC 4 antibody
    • RIC4 antibody
    • SEC 9 antibody
    • SEC9 antibody
    • SNAP 25 antibody
    • SNAP antibody
    • SNAP-25 antibody
    • SNAP-25B antibody
    • SNAP25 antibody
    • SNP 25 antibody
    • SNP25 antibody
    • SNP25_HUMAN antibody
    • sp antibody
    • SUP antibody
    • Super protein antibody
    • Synaptosomal associated 25 kDa protein antibody
    • Synaptosomal associated protein antibody
    • Synaptosomal associated protein 25 antibody
    • Synaptosomal associated protein 25kDa antibody
    • Synaptosomal-associated 25 kDa protein antibody
    • Synaptosomal-associated protein 25 antibody
    • Synaptosomal-associated protein, 25-KD antibody
    see all

Images

  • Anti-SNAP25 antibody (ab228880) at 1/1000 dilution + Rat brain lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 23 kDa



    12% SDS-PAGE gel.

  • Anti-SNAP25 antibody (ab228880) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 23 kDa



    12% SDS-PAGE gel.

  • Anti-SNAP25 antibody (ab228880) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 23 kDa



    12% SDS-PAGE gel.

  • Paraffin-embedded mouse fore brain tissue stained for SNAP25 using ab228880 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat hind brain tissue stained for SNAP25 using ab228880 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded SW480 xenograft tissue stained for SNAP25 using ab228880 at 1/100 dilution in immunohistochemical analysis.

  • Frozen sectioned E13.5 rat brain tissue stained for SNAP25 using ab228880 at 1/250 dilution in immunohistochemical analysis (green).

    The nuclear counterstain is DAPI (blue). Beta Tubulin 3, a mature neuron marker, is detected with a beta Tubulin 3 antibody at 1/500 dilution (red).

  • 4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons cells stained for SNAP25 (green) using ab228880 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: DAPI (blue). Beta Tubulin 3, a mature neuron marker, is detected with a beta Tubulin 3 antibody at 1/500 dilution (red).

References

ab228880 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228880.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up