Key features and details
- Rabbit polyclonal to SNAP25
- Suitable for: WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Product nameAnti-SNAP25 antibody
See all SNAP25 primary antibodies
DescriptionRabbit polyclonal to SNAP25
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human, Pig
Predicted to work with: Rat, Chicken, Cow, Chimpanzee, Rhesus monkey, Orangutan
Recombinant full length protein (His-tag) corresponding to Human SNAP25 aa 1-206. (Expressed in E.coli). 100% homologous to SNAP25 from Mouse and Rat.
MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVML DEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDA YKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENL EQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRAT KMLGSG
Database link: P60880
- WB: Recombinant human SNAP25 protein; Mouse spinal cord and cerebellum tissue; Pig brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231641 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 23 kDa.|
Functiont-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
Tissue specificityNeurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
Sequence similaritiesBelongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains.
modificationsPalmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
Cellular localizationCytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
- Information by UniProt
- bA416N4.2 antibody
- Bdr antibody
- CMS18 antibody
All lanes : Anti-SNAP25 antibody (ab231641) at 1 µg/ml
Lane 1 : Mouse spinal cord lysate.
Lane 2 : Mouse cerebellum lysate.
Lane 3 : Pig brain lysate.
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 23 kDa
Anti-SNAP25 antibody (ab231641) at 1 µg/ml + Recombinant human SNAP25 protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 23 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231641 has not yet been referenced specifically in any publications.