• Product name
  • Description
    Rabbit polyclonal to SNAP25
  • Host species
  • Tested applications
    Suitable for: IP, IHC-FoFr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
    Predicted to work with: Chicken, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human SNAP25.

    Read Abcam's proprietary immunogen policy (Peptide available as ab41724.)

  • Positive control
    • Recombinant Human SNAP25 protein (ab51048) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: Spinal cord (Rat) tissue lysate and Hippocampus (Mouse) tissue Lysate. ICC/IF: PC12 cell line.



Our Abpromise guarantee covers the use of ab41455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
IHC-FoFr 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • Function
    t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
  • Tissue specificity
    Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
  • Sequence similarities
    Belongs to the SNAP-25 family.
    Contains 2 t-SNARE coiled-coil homology domains.
  • Post-translational
    Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
  • Cellular localization
    Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA416N4.2 antibody
    • Bdr antibody
    • CMS18 antibody
    • dJ1068F16.2 antibody
    • FLJ23079 antibody
    • HGNC:11132 antibody
    • MGC105414 antibody
    • MGC139754 antibody
    • Resistance to inhibitors of cholinesterase 4 homolog antibody
    • RIC 4 antibody
    • RIC4 antibody
    • SEC 9 antibody
    • SEC9 antibody
    • SNAP 25 antibody
    • SNAP antibody
    • SNAP-25 antibody
    • SNAP-25B antibody
    • SNAP25 antibody
    • SNP 25 antibody
    • SNP25 antibody
    • SNP25_HUMAN antibody
    • sp antibody
    • SUP antibody
    • Super protein antibody
    • Synaptosomal associated 25 kDa protein antibody
    • Synaptosomal associated protein antibody
    • Synaptosomal associated protein 25 antibody
    • Synaptosomal associated protein 25kDa antibody
    • Synaptosomal-associated 25 kDa protein antibody
    • Synaptosomal-associated protein 25 antibody
    • Synaptosomal-associated protein, 25-KD antibody
    see all


  • All lanes : Anti-SNAP25 antibody (ab41455) at 1 µg/ml

    Lane 1 : Spinal cord (Rat) tissue lysate
    Lane 2 : Hippocampus (Mouse) tissue Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?

  • Anti-SNAP25 antibody (ab41455) at 1 µg/ml + Recombinant Human SNAP25 protein (ab74529) at 0.01 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa

    Exposure time: 1 minute
  • ab4455 staining SNAP25 in PC12 cells. The cells were fixed with 100% methanol (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab41455 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

  • ab41455 staining SNAP25 in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/3000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary.

    The picture shows the staining obtained at the level of the hippocampus. The staining was very strong, located in the whole brain.

    See Abreview

  • All lanes : Anti-SNAP25 antibody (ab41455) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : SH-SY5Y (Human neuroblastoma cell line) whole cell lysate at 20 µg
    Lane 4 : Mouse brain homogenate at 20 µg

    All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 26 kDa why is the actual band size different from the predicted?

    Exposure time: 2 minutes
  • SNAP25 was immunoprecipitated using 0.5mg Rat Spinal Cord tissue lysate, 5µg of Rabbit polyclonal to SNAP25 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Rat Spinal Cord tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab41455.
    Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
    Band: 26kDa; SNAP25


This product has been referenced in:
  • Pan Y  et al. Pancreatic fibroblast growth factor 21 protects against type 2 diabetes in mice by promoting insulin expression and secretion in a PI3K/Akt signaling-dependent manner. J Cell Mol Med N/A:N/A (2018). Read more (PubMed: 30461198) »
  • Shirafuji T  et al. The Role of Cysteine String Protein a Phosphorylation at Serine 10 and 34 by Protein Kinase C? for Presynaptic Maintenance. J Neurosci 38:278-290 (2018). Read more (PubMed: 29167402) »
See all 9 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Flow Cytometry
Human Cell (Differentiated hNSCs)
Yes - 0.25% Triton X-100 in DPBS
Gating Strategy
Undifferentiated Stem Cells (white)
Differentiated hNSCs
Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS

Abcam user community

Verified customer

Submitted Sep 11 2015


Thank you for your reply and for sending the information. I am sending a vial of ab41455 free of charge on the order ***, and this vial will also be covered by our Abpromise guarantee. If you have any problems with it, please let me know and I will be happy to arrange a refund for you. Let me know if you have any questions or if there is anything else that we can do for you.

Read More


Thank you for contacting us and for letting us know about the trouble with ab53723. I am sorry that the results have not been satisfactory. I do have a couple of questions so that I can better understand the situation: 1) What region of brain was the human sample taken from? SNAP25 is only expressed in certain regions which could account for the difference in signal from the rat cortex samples and the human brain samples. 2) What kind of blocking solution was used? 3) What lysis buffer was used to produce the tissue homogenate? We do guarantee all of our products to work as stated on the datasheet for up to 6 months after purchase, so I will be happy to either send a replacement such as ab41455 or refund the original purchase if the antibody is not working properly. I look forward to hearing from you. Please let me know if you have any questions.

Read More
Immunohistochemistry (PFA perfusion fixed frozen sections)
Rat Tissue sections (Brain sections)
Brain sections
Antigen retrieval step

Dr. Sophie Pezet

Verified customer

Submitted Mar 17 2009

For licensing inquiries, please contact partnerships@abcam.com

Sign up