Western blot abreview for Anti-SNAP25 antibody

Good
Abreviews
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Non-reduced Denaturing (Extra PAGE ONE 5-20%)
Sample
Chicken Tissue lysate - whole (Brain and DRG)
Specification
Brain and DRG
Blocking step
Blocking one as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Can Get Signal
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Rabbit IgG, HRP-Linked Whole Ab Donkey
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ImmunoStar Zeta
Bands
Specific: 25 kDa
Exposure
1 minute(s) and 0 second(s)

Abcam user community

Verified customer

Submitted Feb 09 2015

Sign up