Overview

  • Product name

    Anti-SNAP25 antibody - C-terminal (Biotin)
    See all SNAP25 primary antibodies
  • Description

    Goat polyclonal to SNAP25 - C-terminal (Biotin)
  • Host species

    Goat
  • Conjugation

    Biotin
  • Specificity

    ab214783 is expected to recognise both reported isoforms NP_003072.2 (SNAP25A) and NP_570824.1 (SNAP25B).
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Chicken, Cow, Human, Chimpanzee, Zebrafish, Rhesus monkey, Goldfish, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human SNAP25 aa 193-206 (C terminal) (Cysteine residue). NP_003072.2
    Sequence:

    DEANQRATKMLGSG


    Database link: P60880

  • Positive control

    • Rat brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab214783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23.3 kDa).

Target

  • Function

    t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
  • Tissue specificity

    Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
  • Sequence similarities

    Belongs to the SNAP-25 family.
    Contains 2 t-SNARE coiled-coil homology domains.
  • Post-translational
    modifications

    Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
  • Cellular localization

    Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • bA416N4.2 antibody
    • Bdr antibody
    • CMS18 antibody
    • dJ1068F16.2 antibody
    • FLJ23079 antibody
    • HGNC:11132 antibody
    • MGC105414 antibody
    • MGC139754 antibody
    • Resistance to inhibitors of cholinesterase 4 homolog antibody
    • RIC 4 antibody
    • RIC-4 antibody
    • RIC4 antibody
    • SEC 9 antibody
    • SEC9 antibody
    • SNAP 25 antibody
    • SNAP antibody
    • SNAP-25 antibody
    • SNAP-25B antibody
    • SNAP25 antibody
    • SNP 25 antibody
    • SNP25 antibody
    • SNP25_HUMAN antibody
    • sp antibody
    • SUP antibody
    • Super protein antibody
    • Synaptosomal associated 25 kDa protein antibody
    • Synaptosomal associated protein antibody
    • Synaptosomal associated protein 25 antibody
    • Synaptosomal associated protein 25kDa antibody
    • Synaptosomal-associated 25 kDa protein antibody
    • Synaptosomal-associated protein 25 antibody
    • Synaptosomal-associated protein antibody
    • Synaptosomal-associated protein, 25-KD antibody
    see all

Images

  • Anti-SNAP25 antibody - C-terminal (Biotin) (ab214783) at 0.1 µg/ml + Rat Brain lysate in RIPA buffer at 35 µg

    Secondary
    streptavidin-HRP in NAP blocker

    Developed using the ECL technique.

    Predicted band size: 23.3 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?

References

ab214783 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab214783.
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