Product nameAnti-SNAP25 antibody [EP3274]
See all SNAP25 primary antibodies
DescriptionRabbit monoclonal [EP3274] to SNAP25
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)
- Fetal brain, HeLa, SH-SY5Y, U87-MG, fetal hippocampus, fetal cerebellum, and Neuro-2a cell lysates; Human brain tissue; Human cerebellum tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab108990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).|
|IHC-P||1/100 - 1/250. Antigen retrieval is recommended.|
|ICC||1/100 - 1/250.|
Functiont-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
Tissue specificityNeurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
Sequence similaritiesBelongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains.
modificationsPalmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
Cellular localizationCytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
- Information by UniProt
- bA416N4.2 antibody
- Bdr antibody
- CMS18 antibody
ab108990, at 1/100 dilution, staining SNAP25 in paraffin-embedded Human brain tissue by Immunohistochemistry.
ab108990, at 1/100 dilution, staining SNAP25 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
All lanes : Anti-SNAP25 antibody [EP3274] (ab108990) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : U87-MG cell lysate
Lane 5 : Fetal hippocampus lysate
Lane 6 : Fetal cerebellum lysate
Lane 7 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa
ab108990 showing negative staining in Normal colon tissue.
ab108990 showing negative staining in Normal spleen tissue.
ab108990 showing negative staining in Skeletal muscle tissue.
This product has been referenced in:
- Shi TY et al. Kainate receptor mediated presynaptic LTP in agranular insular cortex contributes to fear and anxiety in mice. Neuropharmacology 128:388-400 (2018). WB . Read more (PubMed: 29104074) »
- Almandoz-Gil L et al. In Situ Proximity Ligation Assay Reveals Co-Localization of Alpha-Synuclein and SNARE Proteins in Murine Primary Neurons. Front Neurol 9:180 (2018). Mouse . Read more (PubMed: 29623065) »