Overview

  • Product name

    Anti-SNAP25 antibody [EP3274]
    See all SNAP25 primary antibodies
  • Description

    Rabbit monoclonal [EP3274] to SNAP25
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • Positive control

    • Fetal brain, HeLa, SH-SY5Y, U87-MG, fetal hippocampus, fetal cerebellum, and Neuro-2a cell lysates; Human brain tissue; Human cerebellum tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
    • Tissue specificity

      Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
    • Sequence similarities

      Belongs to the SNAP-25 family.
      Contains 2 t-SNARE coiled-coil homology domains.
    • Post-translational
      modifications

      Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
    • Cellular localization

      Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
    • Information by UniProt
    • Database links

    • Alternative names

      • bA416N4.2 antibody
      • Bdr antibody
      • CMS18 antibody
      • dJ1068F16.2 antibody
      • FLJ23079 antibody
      • HGNC:11132 antibody
      • MGC105414 antibody
      • MGC139754 antibody
      • Resistance to inhibitors of cholinesterase 4 homolog antibody
      • RIC 4 antibody
      • RIC-4 antibody
      • RIC4 antibody
      • SEC 9 antibody
      • SEC9 antibody
      • SNAP 25 antibody
      • SNAP antibody
      • SNAP-25 antibody
      • SNAP-25B antibody
      • SNAP25 antibody
      • SNP 25 antibody
      • SNP25 antibody
      • SNP25_HUMAN antibody
      • sp antibody
      • SUP antibody
      • Super protein antibody
      • Synaptosomal associated 25 kDa protein antibody
      • Synaptosomal associated protein antibody
      • Synaptosomal associated protein 25 antibody
      • Synaptosomal associated protein 25kDa antibody
      • Synaptosomal-associated 25 kDa protein antibody
      • Synaptosomal-associated protein 25 antibody
      • Synaptosomal-associated protein antibody
      • Synaptosomal-associated protein, 25-KD antibody
      see all

    Images

    • ab108990, at 1/100 dilution, staining SNAP25 in paraffin-embedded Human brain tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab108990, at 1/100 dilution, staining SNAP25 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • All lanes : Anti-SNAP25 antibody [EP3274] (ab108990) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : U87-MG cell lysate
      Lane 5 : Fetal hippocampus lysate
      Lane 6 : Fetal cerebellum lysate
      Lane 7 : Neuro-2a cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 23 kDa

    • ab108990 showing negative staining in human Normal colon tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab108990 showing negative staining in human Normal spleen tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab108990 showing negative staining in human Skeletal muscle tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Shi TY  et al. Kainate receptor mediated presynaptic LTP in agranular insular cortex contributes to fear and anxiety in mice. Neuropharmacology 128:388-400 (2018). WB . Read more (PubMed: 29104074) »
    • Almandoz-Gil L  et al. In Situ Proximity Ligation Assay Reveals Co-Localization of Alpha-Synuclein and SNARE Proteins in Murine Primary Neurons. Front Neurol 9:180 (2018). Mouse . Read more (PubMed: 29623065) »
    See all 5 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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