Recombinant
RabMAb

Recombinant Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)

Overview

  • Product name

    Anti-SNAP25 antibody [EP3274] - BSA and Azide free
    See all SNAP25 primary antibodies
  • Description

    Rabbit monoclonal [EP3274] to SNAP25 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SNAP25 aa 100-200 (internal sequence). The exact sequence is proprietary.
    (Peptide available as ab183130)

  • General notes

    Ab226046 is the carrier-free version of ab108990. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab226046 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab226046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Antigen retrieval is recommended.

WB Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
    • Tissue specificity

      Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
    • Sequence similarities

      Belongs to the SNAP-25 family.
      Contains 2 t-SNARE coiled-coil homology domains.
    • Post-translational
      modifications

      Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
    • Cellular localization

      Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
    • Information by UniProt
    • Database links

    • Alternative names

      • bA416N4.2 antibody
      • Bdr antibody
      • CMS18 antibody
      • dJ1068F16.2 antibody
      • FLJ23079 antibody
      • HGNC:11132 antibody
      • MGC105414 antibody
      • MGC139754 antibody
      • Resistance to inhibitors of cholinesterase 4 homolog antibody
      • RIC 4 antibody
      • RIC-4 antibody
      • RIC4 antibody
      • SEC 9 antibody
      • SEC9 antibody
      • SNAP 25 antibody
      • SNAP antibody
      • SNAP-25 antibody
      • SNAP-25B antibody
      • SNAP25 antibody
      • SNP 25 antibody
      • SNP25 antibody
      • SNP25_HUMAN antibody
      • sp antibody
      • SUP antibody
      • Super protein antibody
      • Synaptosomal associated 25 kDa protein antibody
      • Synaptosomal associated protein antibody
      • Synaptosomal associated protein 25 antibody
      • Synaptosomal associated protein 25kDa antibody
      • Synaptosomal-associated 25 kDa protein antibody
      • Synaptosomal-associated protein 25 antibody
      • Synaptosomal-associated protein antibody
      • Synaptosomal-associated protein, 25-KD antibody
      see all

    Images

    • ab108990, at 1/100 dilution, staining SNAP25 in paraffin-embedded Human brain tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108990).

    • ab108990, at 1/100 dilution, staining SNAP25 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108990).

    • ab108990 showing negative staining in Normal colon tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108990).

    • ab108990 showing negative staining in Normal spleen tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108990).

    • ab108990 showing negative staining in Skeletal muscle tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108990).

    References

    ab226046 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab226046.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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