Anti-SNAP25 antibody [EPR3275] (ab109105)
- Datasheet
- References (6)
- Protocols
Overview
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Product nameAnti-SNAP25 antibody [EPR3275]
See all SNAP25 primary antibodies -
DescriptionRabbit monoclonal [EPR3275] to SNAP25
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human SNAP25. The exact sequence is proprietary.
(Peptide available asab183131) -
Positive control
- WB: Human fetal brain tissue lysate and SH SY5Y, and Neuro 2a cell lysates. ICC/IF: SH SY5Y cells. Flow Cyt: SH-SY5Y cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR3275
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab109105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 23 kDa. | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/200. | |
| ICC/IF | 1/100 - 1/250. |
Target
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Functiont-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
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Tissue specificityNeurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
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Sequence similaritiesBelongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains. -
Post-translational
modificationsPalmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association. -
Cellular localizationCytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
- Information by UniProt
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Database links
- Entrez Gene: 6616 Human
- Entrez Gene: 20614 Mouse
- Entrez Gene: 25012 Rat
- Omim: 600322 Human
- SwissProt: P60880 Human
- SwissProt: P60879 Mouse
- SwissProt: P60881 Rat
- Unigene: 167317 Human
see all -
Alternative names
- bA416N4.2 antibody
- Bdr antibody
- CMS18 antibody
see all
Images
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![Western blot - Anti-SNAP25 antibody [EPR3275] (ab109105)](https://www.abcam.com/ps/products/109/ab109105/Images/SNAP25-Primary-antibodies-ab109105-1.JPG)
Western blot - Anti-SNAP25 antibody [EPR3275] (ab109105)All lanes : Anti-SNAP25 antibody [EPR3275] (ab109105) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : SH SY5Y lysate
Lane 3 : Neuro 2a lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody [EPR3275] (ab109105)](https://www.abcam.com/ps/products/109/ab109105/Images/SNAP25-Primary-antibodies-ab109105-2.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody [EPR3275] (ab109105)Immunofluorescent staining of SNAP25 in SH SY5Y cells using ab109105 at 1/100. -
![Flow Cytometry - Anti-SNAP25 antibody [EPR3275] (ab109105)](https://www.abcam.com/ps/products/109/ab109105/Images/ab109105-3-ab109105FC1.jpg)
Flow Cytometry - Anti-SNAP25 antibody [EPR3275] (ab109105)Flow cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling SNAP25 (red) with ab109105 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Huang CJ et al. Detection of lower levels of SNAP25 using multiple microarray systems and its functional significance in medulloblastoma. Int J Mol Med 39:1195-1205 (2017). IF ; Human . Read more (PubMed: 28339008) »
- Geerts CJ et al. Tomosyn associates with secretory vesicles in neurons through its N- and C-terminal domains. PLoS One 12:e0180912 (2017). Read more (PubMed: 28746398) »
