Recombinant
RabMAb

Recombinant Anti-SNAP25 antibody [EPR3275] (ab109105)

Reviews (1)Q&A (1)References (9)

Overview

  • Product name

    Anti-SNAP25 antibody [EPR3275]
    See all SNAP25 primary antibodies

  • Description

    Rabbit monoclonal [EPR3275] to SNAP25

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SNAP25. The exact sequence is proprietary.
    (Peptide available as ab183131)

  • Positive control

    • WB: Human fetal brain tissue lysate and SH SY5Y, and Neuro 2a cell lysates. ICC/IF: SH SY5Y cells. Flow Cyt: SH-SY5Y cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab109105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/200.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.

    • Tissue specificity

      Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.

    • Sequence similarities

      Belongs to the SNAP-25 family.
      Contains 2 t-SNARE coiled-coil homology domains.

    • Post-translational
      modifications

      Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.

    • Cellular localization

      Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • bA416N4.2 antibody
      • Bdr antibody
      • CMS18 antibody
      • dJ1068F16.2 antibody
      • FLJ23079 antibody
      • HGNC:11132 antibody
      • MGC105414 antibody
      • MGC139754 antibody
      • Resistance to inhibitors of cholinesterase 4 homolog antibody
      • RIC 4 antibody
      • RIC-4 antibody
      • RIC4 antibody
      • SEC 9 antibody
      • SEC9 antibody
      • SNAP 25 antibody
      • SNAP antibody
      • SNAP-25 antibody
      • SNAP-25B antibody
      • SNAP25 antibody
      • SNP 25 antibody
      • SNP25 antibody
      • SNP25_HUMAN antibody
      • sp antibody
      • SUP antibody
      • Super protein antibody
      • Synaptosomal associated 25 kDa protein antibody
      • Synaptosomal associated protein antibody
      • Synaptosomal associated protein 25 antibody
      • Synaptosomal associated protein 25kDa antibody
      • Synaptosomal-associated 25 kDa protein antibody
      • Synaptosomal-associated protein 25 antibody
      • Synaptosomal-associated protein antibody
      • Synaptosomal-associated protein, 25-KD antibody
      see all

    Images

    • Western blot - Anti-SNAP25 antibody [EPR3275] (ab109105)
      Western blot - Anti-SNAP25 antibody [EPR3275] (ab109105)
      All lanes : Anti-SNAP25 antibody [EPR3275] (ab109105) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : SH SY5Y lysate
      Lane 3 : Neuro 2a lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 23 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody [EPR3275] (ab109105)
      Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody [EPR3275] (ab109105)
      Immunofluorescent staining of SNAP25 in SH SY5Y cells using ab109105 at 1/100.
    • Flow Cytometry - Anti-SNAP25 antibody [EPR3275] (ab109105)
      Flow Cytometry - Anti-SNAP25 antibody [EPR3275] (ab109105)

      Flow cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling SNAP25 (red) with ab109105 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    References

    This product has been referenced in:

    • Cui Z  et al. Nanodiamond autophagy inhibitor allosterically improves the arsenical-based therapy of solid tumors. Nat Commun 9:4347 (2018). Read more (PubMed: 30341298) »
    • Lizarbe B  et al. Neurochemical Modifications in the Hippocampus, Cortex and Hypothalamus of Mice Exposed to Long-Term High-Fat Diet. Front Neurosci 12:985 (2018). Read more (PubMed: 30670942) »
    See all 9 Publications for this product

    Publishing research using ab109105? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-SNAP25 antibody [EPR3275]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Purified protein (Synaptosomes)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (4–20% Mini-PROTEAN TGX Precast Protein Gel)
    Loading amount
    10 µg
    Specification
    Synaptosomes
    Blocking step
    Odyssey Blocking Buffer (PBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 15 2019

    Question

    Hi, I am wondering if you could provide us with the information on the epitope sequence/region of SNAP-25 that this antibody recognizes (eg. N-terminus, C-terminus..).

    Read More
    Answer

    Thank you for contacting us.

    The peptide maps between amino acids 180 - 206 of human SNAP-25, at the C-terminus (UniProt: P60880).

    Please let me know if you have any questions.


    We hope this response has sufficiently answered your questions.

    Read More

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