Recombinant
RabMAb

Anti-SNAP25 antibody [EPR3275] (ab109105)

Submit an Abreview Q&A (1)Specific References (6)

Overview

  • Product name
    Anti-SNAP25 antibody [EPR3275]
    See all SNAP25 primary antibodies
  • Description
    Rabbit monoclonal [EPR3275] to SNAP25
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SNAP25. The exact sequence is proprietary.
    (Peptide available as ab183131)

  • Positive control
    • WB: Human fetal brain tissue lysate and SH SY5Y, and Neuro 2a cell lysates. ICC/IF: SH SY5Y cells. Flow Cyt: SH-SY5Y cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/200.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function
      t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
    • Tissue specificity
      Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
    • Sequence similarities
      Belongs to the SNAP-25 family.
      Contains 2 t-SNARE coiled-coil homology domains.
    • Post-translational
      modifications
      Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
    • Cellular localization
      Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • bA416N4.2 antibody
      • Bdr antibody
      • CMS18 antibody
      • dJ1068F16.2 antibody
      • FLJ23079 antibody
      • HGNC:11132 antibody
      • MGC105414 antibody
      • MGC139754 antibody
      • Resistance to inhibitors of cholinesterase 4 homolog antibody
      • RIC 4 antibody
      • RIC4 antibody
      • SEC 9 antibody
      • SEC9 antibody
      • SNAP 25 antibody
      • SNAP antibody
      • SNAP-25 antibody
      • SNAP-25B antibody
      • SNAP25 antibody
      • SNP 25 antibody
      • SNP25 antibody
      • SNP25_HUMAN antibody
      • sp antibody
      • SUP antibody
      • Super protein antibody
      • Synaptosomal associated 25 kDa protein antibody
      • Synaptosomal associated protein antibody
      • Synaptosomal associated protein 25 antibody
      • Synaptosomal associated protein 25kDa antibody
      • Synaptosomal-associated 25 kDa protein antibody
      • Synaptosomal-associated protein 25 antibody
      • Synaptosomal-associated protein, 25-KD antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody [EPR3275] (ab109105)
      Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody [EPR3275] (ab109105)
      Immunofluorescent staining of SNAP25 in SH SY5Y cells using ab109105 at 1/100.
    • Western blot - Anti-SNAP25 antibody [EPR3275] (ab109105)
      Western blot - Anti-SNAP25 antibody [EPR3275] (ab109105)
      All lanes : Anti-SNAP25 antibody [EPR3275] (ab109105) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : SH SY5Y lysate
      Lane 3 : Neuro 2a lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 23 kDa

    • Flow Cytometry - Anti-SNAP25 antibody [EPR3275] (ab109105)
      Flow Cytometry - Anti-SNAP25 antibody [EPR3275] (ab109105)

      Flow cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling SNAP25 (red) with ab109105 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    References

    This product has been referenced in:
    • Huang CJ  et al. Detection of lower levels of SNAP25 using multiple microarray systems and its functional significance in medulloblastoma. Int J Mol Med 39:1195-1205 (2017). IF ; Human . Read more (PubMed: 28339008) »
    • Geerts CJ  et al. Tomosyn associates with secretory vesicles in neurons through its N- and C-terminal domains. PLoS One 12:e0180912 (2017). Read more (PubMed: 28746398) »

    See all 6 Publications for this product

    Publishing research using ab109105? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    1 Review or Q&A

    10
    Votes: Highest First

    Hi, I am wondering if you could provide us with the information on the epitope sequence/region of SNAP-25 that this antibody recognizes (eg. N-terminus, C-terminus..).

    Thank you for contacting us.

    The peptide maps between amino acids 180 - 206 of human SNAP-25, at the C-terminus (UniProt: P60880).

    Please let me know if you have any questions.


    We hope this response has sufficiently answ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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