Recombinant Anti-SNAP25 antibody [EPR3275] (ab109105)
![Western blot - Anti-SNAP25 antibody [EPR3275] (ab109105)](https://www.abcam.com/ps/products/109/ab109105/Images/ab109105-121670-anti-snap25-antibody-epr3275-western-blot.jpg "Western blot - Anti-SNAP25 antibody [EPR3275] (ab109105)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3275] to SNAP25
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
Overview
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Product name
Anti-SNAP25 antibody [EPR3275]
See all SNAP25 primary antibodies -
Description
Rabbit monoclonal [EPR3275] to SNAP25 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain tissue lysate and SH SY5Y, and Neuro 2a cell lysates. ICC/IF: SH SY5Y cells. Flow Cyt: SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3275 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
|
| Flow Cyt |
1/200.
|
|
| ICC/IF |
1/100 - 1/250.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa. |
|
Flow Cyt
1/200. |
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ICC/IF
1/100 - 1/250. |
Application notes
Is unsuitable for IHC-P.
Target
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Function
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. -
Tissue specificity
Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. -
Sequence similarities
Belongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains. -
Post-translational
modificationsPalmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association. -
Cellular localization
Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 6616 Human
- Entrez Gene: 20614 Mouse
- Entrez Gene: 25012 Rat
- Omim: 600322 Human
- SwissProt: P60880 Human
- SwissProt: P60879 Mouse
- SwissProt: P60881 Rat
- Unigene: 167317 Human
see all -
Alternative names
- bA416N4.2 antibody
- Bdr antibody
- CMS18 antibody
see all
Images
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Western blot - Anti-SNAP25 antibody [EPR3275] (ab109105)All lanes : Anti-SNAP25 antibody [EPR3275] (ab109105) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : SH SY5Y lysate
Lane 3 : Neuro 2a lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody [EPR3275] (ab109105)](https://www.abcam.com/ps/products/109/ab109105/Images/ab109105-121669-anti-snap25-antibody-epr3275-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody [EPR3275] (ab109105)Immunofluorescent staining of SNAP25 in SH SY5Y cells using ab109105 at 1/100. -
![Flow Cytometry - Anti-SNAP25 antibody [EPR3275] (ab109105)](https://www.abcam.com/ps/products/109/ab109105/Images/ab109105-280389-anti-snap25-antibody-epr3275-flow-cytometry.jpg)
Flow Cytometry - Anti-SNAP25 antibody [EPR3275] (ab109105)Flow cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling SNAP25 (red) with ab109105 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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![Anti-SNAP25 antibody [EPR3275] (ab109105)](https://www.abcam.com/ps/products/109/ab109105/Images/ab109105-4-benefits-of-recombinant-antibodies.png)
Anti-SNAP25 antibody [EPR3275] (ab109105)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab109105 has been referenced in 9 publications.
- Cui Z et al. Nanodiamond autophagy inhibitor allosterically improves the arsenical-based therapy of solid tumors. Nat Commun 9:4347 (2018). PubMed: 30341298
- Lizarbe B et al. Neurochemical Modifications in the Hippocampus, Cortex and Hypothalamus of Mice Exposed to Long-Term High-Fat Diet. Front Neurosci 12:985 (2018). PubMed: 30670942
- Mendsaikhan A et al. Differences in Gene Expression Profiles and Phenotypes of Differentiated SH-SY5Y Neurons Stably Overexpressing Mitochondrial Ferritin. Front Mol Neurosci 11:470 (2018). PubMed: 30670947
- Huang CJ et al. Detection of lower levels of SNAP25 using multiple microarray systems and its functional significance in medulloblastoma. Int J Mol Med 39:1195-1205 (2017). IF ; Human . PubMed: 28339008
- Geerts CJ et al. Tomosyn associates with secretory vesicles in neurons through its N- and C-terminal domains. PLoS One 12:e0180912 (2017). PubMed: 28746398
- Wang C et al. Proteome Analysis of Potential Synaptic Vesicle Cycle Biomarkers in the Cerebrospinal Fluid of Patients with Sporadic Creutzfeldt-Jakob Disease. Mol Neurobiol 54:5177-5191 (2017). WB . PubMed: 27562179
- Yadirgi G et al. Immuno-detection of cleaved SNAP-25 from differentiated mouse embryonic stem cells provides a sensitive assay for determination of botulinum A toxin and antitoxin potency. J Immunol Methods 451:90-99 (2017). WB . PubMed: 28943257
- Mu Y et al. NUPR1 maintains autolysosomal efflux by activating SNAP25 transcription in cancer cells. Autophagy N/A:1-17 (2017). PubMed: 29130426
- Cheng YC et al. Knocking down of heat-shock protein 27 directs differentiation of functional glutamatergic neurons from placenta-derived multipotent cells. Sci Rep 6:30314 (2016). ICC/IF ; Human . PubMed: 27444754