Key features and details
- Rabbit polyclonal to SNAP29 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SNAP29 antibody - C-terminal
See all SNAP29 primary antibodies
DescriptionRabbit polyclonal to SNAP29 - C-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rhesus monkey
Recombinant fragment within Human SNAP29 (C terminal). The exact sequence is proprietary.
Database link: O95721
- WB: Mouse and rat brain tissue extracts; HEK-293T, A431, HeLa and HepG2 whole cell extracts. ICC/IF: DIV9 rat E18 primary cortical neurons.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 29 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionInvolved in multiple membrane trafficking steps.
Tissue specificityFound in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas.
Involvement in diseaseDefects in SNAP29 are the cause of CEDNIK syndrome (CEDNIK) [MIM:609528]. CEDNIK is a neurocutaneous syndrome characterized by cerebral dysgenesis, neuropathy, ichthyosis and palmoplantar keratoderma.
Sequence similaritiesBelongs to the SNAP-25 family.
Contains 1 t-SNARE coiled-coil homology domain.
Cellular localizationCytoplasm. Membrane. Cell junction > synapse > synaptosome. Appears to be mostly membrane-bound, probably via interaction with syntaxins, but a significant portion is cytoplasmic.
- Information by UniProt
- CEDNIK antibody
- FLJ21051 antibody
- SNAP 29 antibody
All lanes : Anti-SNAP29 antibody - C-terminal (ab229584) at 1/500 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.
Predicted band size: 29 kDa
12% SDS-PAGE gel.
4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for SNAP29 (green) using ab229584 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Fluoroshield with DAPI (blue). beta Tubulin 3/ Tuj1, a neuron cell marker, stained by anti-beta Tubulin 3/ Tuj1 antibody at 1/500 dilution.
All lanes : Anti-SNAP29 antibody - C-terminal (ab229584) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229584 has not yet been referenced specifically in any publications.