Overview

  • Product name

    Anti-SNAP29 antibody - C-terminal
    See all SNAP29 primary antibodies
  • Description

    Rabbit polyclonal to SNAP29 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human SNAP29 (C terminal). The exact sequence is proprietary.
    Database link: O95721

  • Positive control

    • WB: Mouse and rat brain tissue extracts; HEK-293T, A431, HeLa and HepG2 whole cell extracts. ICC/IF: DIV9 rat E18 primary cortical neurons.

Properties

Applications

Our Abpromise guarantee covers the use of ab229584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Involved in multiple membrane trafficking steps.
  • Tissue specificity

    Found in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas.
  • Involvement in disease

    Defects in SNAP29 are the cause of CEDNIK syndrome (CEDNIK) [MIM:609528]. CEDNIK is a neurocutaneous syndrome characterized by cerebral dysgenesis, neuropathy, ichthyosis and palmoplantar keratoderma.
  • Sequence similarities

    Belongs to the SNAP-25 family.
    Contains 1 t-SNARE coiled-coil homology domain.
  • Cellular localization

    Cytoplasm. Membrane. Cell junction > synapse > synaptosome. Appears to be mostly membrane-bound, probably via interaction with syntaxins, but a significant portion is cytoplasmic.
  • Information by UniProt
  • Database links

  • Alternative names

    • CEDNIK antibody
    • FLJ21051 antibody
    • SNAP 29 antibody
    • SNAP-29 antibody
    • SNAP29 antibody
    • SNP29_HUMAN antibody
    • Soluble 29 kDa NSF attachment protein antibody
    • Synaptosomal associated protein 29 antibody
    • Synaptosomal associated protein 29kDa antibody
    • Synaptosomal-associated protein 29 antibody
    • Vesicle membrane fusion protein SNAP 29 antibody
    • Vesicle membrane fusion protein SNAP29 antibody
    • Vesicle-membrane fusion protein SNAP-29 antibody
    see all

Images

  • All lanes : Anti-SNAP29 antibody - C-terminal (ab229584) at 1/500 dilution

    Lane 1 : Mouse brain tissue extract
    Lane 2 : Rat brain tissue extract

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 29 kDa



    12% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for SNAP29 (green) using ab229584 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Fluoroshield with DAPI (blue). beta Tubulin 3/ Tuj1, a neuron cell marker, stained by anti-beta Tubulin 3/ Tuj1 antibody at 1/500 dilution.

  • All lanes : Anti-SNAP29 antibody - C-terminal (ab229584) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 29 kDa



    12% SDS-PAGE gel.

References

ab229584 has not yet been referenced specifically in any publications.

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