Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

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Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
  • Immunocytochemistry/ Immunofluorescence - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
  • Flow Cytometry (Intracellular) - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
  • Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
  • Immunoprecipitation - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
  • Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9198(2)] to SNAP29
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-SNAP29 antibody [EPR9198(2)]
    See all SNAP29 primary antibodies

  • Description

    Rabbit monoclonal [EPR9198(2)] to SNAP29

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2, 293 and Jurkat whole cell lysates. ICC/IF: HeLa cells. Flow Cyt (intra): Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ICC/IF
1/100 - 1/250.
IP
1/30 - 1/50.
Notes
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ICC/IF
1/100 - 1/250.
IP
1/30 - 1/50.

Target

  • Function

    Involved in multiple membrane trafficking steps.

  • Tissue specificity

    Found in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas.

  • Involvement in disease

    Defects in SNAP29 are the cause of CEDNIK syndrome (CEDNIK) [MIM:609528]. CEDNIK is a neurocutaneous syndrome characterized by cerebral dysgenesis, neuropathy, ichthyosis and palmoplantar keratoderma.

  • Sequence similarities

    Belongs to the SNAP-25 family.
    Contains 1 t-SNARE coiled-coil homology domain.

  • Cellular localization

    Cytoplasm. Membrane. Cell junction > synapse > synaptosome. Appears to be mostly membrane-bound, probably via interaction with syntaxins, but a significant portion is cytoplasmic.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CEDNIK antibody
    • FLJ21051 antibody
    • SNAP 29 antibody
    • SNAP-29 antibody
    • SNAP29 antibody
    • SNP29_HUMAN antibody
    • Soluble 29 kDa NSF attachment protein antibody
    • Synaptosomal associated protein 29 antibody
    • Synaptosomal associated protein 29kDa antibody
    • Synaptosomal-associated protein 29 antibody
    • Vesicle membrane fusion protein SNAP 29 antibody
    • Vesicle membrane fusion protein SNAP29 antibody
    • Vesicle-membrane fusion protein SNAP-29 antibody
    see all

Images

  • Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    All lanes : Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SNAP29 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab181151 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab181151 Anti-SNAP29 antibody [EPR9198(2)] was shown to specifically react with SNAP29 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265289 (knockout cell lysate ab257693) was used.  Wild-type and SNAP29 knockout samples were subjected to SDS-PAGE.  ab181151 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    Immunocytochemistry/ Immunofluorescence - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

    Immunofluorescent analysis of -20℃ acetone fixed Hela cells staining SNAP29 using ab181151 at 1/250 dilution and Alexa Fluor®555 stained Goat anti Rabbit IgG at 1/250 as a secondary antibody (red). Dapi counterstain (blue).

  • Flow Cytometry (Intracellular) - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    Flow Cytometry (Intracellular) - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

    Flow cytometric analysis of 2% paraformaldehyde fixed Jurkat cells staining SNAP29 using ab181151 at 1/10 dilution, and FITC conjugated Goat anti rabbit IgG at 1/150 as a secondary antibody (red curve). Rabbit monoclonal IgG was used as the negative control (green curve).

  • Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    All lanes : Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/5000 dilution

    Lane 1 : Hela cell lysate with NFDM/TBST
    Lane 2 : 293 cell lysate with NFDM/TBST
    Lane 3 : Jurkat cell lysate with NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

  • Immunoprecipitation - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    Immunoprecipitation - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

    Western blot analysis of SNAP29 in immunoprecipitation pellets from Jurkat cell lysate, using ab181151 at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as a secondary antibody at 1/1000 dilution. Blocking/dilution buffer and concentration: 5% NFDM/TBST



    Lane 1 : Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/50 dilution


    Lane 1 : Precipitate from Jurkat lysate using ab181151 with NFDM/TBST
    Lane 2 : Jurkat lysate with NFDM/TBST

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution
  • Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

References (1)

Publishing research using ab181151? Please let us know so that we can cite the reference in this datasheet.

ab181151 has been referenced in 1 publication.

  • Huang L  et al. O-GlcNAc-modified SNAP29 inhibits autophagy-mediated degradation via the disturbed SNAP29-STX17-VAMP8 complex and exacerbates myocardial injury in type I diabetic rats. Int J Mol Med 42:3278-3290 (2018). PubMed: 30221662

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