Recombinant Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
![Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)](https://www.abcam.com/ps/products/181/ab181151/Images/ab181151-393555-anti-snap29-antibody-epr91982-western-blot-human-knockout-lysate.jpg "Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9198(2)] to SNAP29
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SNAP29 antibody [EPR9198(2)]
See all SNAP29 primary antibodies -
Description
Rabbit monoclonal [EPR9198(2)] to SNAP29 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HepG2, 293 and Jurkat whole cell lysates. ICC/IF: HeLa cells. Flow Cyt (intra): Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9198(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
|
| ICC/IF |
1/100 - 1/250.
|
|
| IP |
1/30 - 1/50.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
|
ICC/IF
1/100 - 1/250. |
|
IP
1/30 - 1/50. |
Target
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Function
Involved in multiple membrane trafficking steps. -
Tissue specificity
Found in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas. -
Involvement in disease
Defects in SNAP29 are the cause of CEDNIK syndrome (CEDNIK) [MIM:609528]. CEDNIK is a neurocutaneous syndrome characterized by cerebral dysgenesis, neuropathy, ichthyosis and palmoplantar keratoderma. -
Sequence similarities
Belongs to the SNAP-25 family.
Contains 1 t-SNARE coiled-coil homology domain. -
Cellular localization
Cytoplasm. Membrane. Cell junction > synapse > synaptosome. Appears to be mostly membrane-bound, probably via interaction with syntaxins, but a significant portion is cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 9342 Human
- Entrez Gene: 67474 Mouse
- Entrez Gene: 116500 Rat
- Omim: 604202 Human
- SwissProt: O95721 Human
- SwissProt: Q9ERB0 Mouse
- SwissProt: Q9Z2P6 Rat
- Unigene: 108002 Human
see all -
Alternative names
- CEDNIK antibody
- FLJ21051 antibody
- SNAP 29 antibody
see all
Images
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Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)All lanes : Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SNAP29 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDaLanes 1-3: Merged signal (red and green). Green - ab181151 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.
ab181151 Anti-SNAP29 antibody [EPR9198(2)] was shown to specifically react with SNAP29 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265289 (knockout cell lysate ab257693) was used. Wild-type and SNAP29 knockout samples were subjected to SDS-PAGE. ab181151 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)](https://www.abcam.com/ps/products/181/ab181151/Images/ab181151-216144-anti-snap29-antibody-epr91982-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)Immunofluorescent analysis of -20°C acetone fixed Hela cells staining SNAP29 using ab181151 at 1/250 dilution and Alexa Fluor® 555 stained Goat anti Rabbit IgG at 1/250 as a secondary antibody (red). Dapi counterstain (blue).
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![Flow Cytometry (Intracellular) - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)](https://www.abcam.com/ps/products/181/ab181151/Images/ab181151-216143-anti-snap29-antibody-epr91982-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)Intracellular flow cytometric analysis of 2% paraformaldehyde fixed Jurkat cells staining SNAP29 using ab181151 at 1/10 dilution, and FITC conjugated Goat anti rabbit IgG at 1/150 as a secondary antibody (red curve). Rabbit monoclonal IgG was used as the negative control (green curve).
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![Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)](https://www.abcam.com/ps/products/181/ab181151/Images/ab181151-216146-anti-snap29-antibody-epr91982-western-blot.jpg)
Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)All lanes : Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/5000 dilution
Lane 1 : HeLa cell lysate with NFDM/TBST
Lane 2 : 293 cell lysate with NFDM/TBST
Lane 3 : Jurkat cell lysate with NFDM/TBST
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa -
![Immunoprecipitation - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)](https://www.abcam.com/ps/products/181/ab181151/Images/ab181151-216145-anti-snap29-antibody-epr91982-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)Western blot analysis of SNAP29 in immunoprecipitation pellets from Jurkat cell lysate, using ab181151 at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as a secondary antibody at 1/1000 dilution. Blocking/dilution buffer and concentration: 5% NFDM/TBST
Lane 1 : Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/50 dilution
Lane 1 : Precipitate from Jurkat lysate using ab181151 with NFDM/TBST
Lane 2 : Jurkat lysate with NFDM/TBST
Blocking peptides at 5 % per lane.
Secondary
All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution -
![Anti-SNAP29 antibody [EPR9198(2)] (ab181151)](https://www.abcam.com/ps/products/181/ab181151/Images/ab181151-6-benefits-of-recombinant-antibodies.png)
Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (4)
ab181151 has been referenced in 4 publications.
- Yan W et al. Decreased neuronal synaptosome associated protein 29 contributes to poststroke cognitive impairment by disrupting presynaptic maintenance. Theranostics 11:4616-4636 (2021). PubMed: 33754017
- Wu W et al. TNF-induced necroptosis initiates early autophagy events via RIPK3-dependent AMPK activation, but inhibits late autophagy. Autophagy N/A:1-18 (2021). PubMed: 33779513
- Chakraborty A et al. SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network. J Cell Sci 133:N/A (2020). PubMed: 32184263
- Huang L et al. O-GlcNAc-modified SNAP29 inhibits autophagy-mediated degradation via the disturbed SNAP29-STX17-VAMP8 complex and exacerbates myocardial injury in type I diabetic rats. Int J Mol Med 42:3278-3290 (2018). PubMed: 30221662
