Recombinant Anti-SNAP29 antibody [EPR9199] (ab138500)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9199] to SNAP29
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SNAP29 antibody [EPR9199]
See all SNAP29 primary antibodies -
Description
Rabbit monoclonal [EPR9199] to SNAP29 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human SNAP29 aa 200-300 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: 293T, Jurkat, HepG2, and HeLa cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 1.30 x 10 -12 M Learn more about KD -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9199 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab138500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (4) |
1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 27 kDa. |
IP
1/10 - 1/100. |
Target
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Function
Involved in multiple membrane trafficking steps. -
Tissue specificity
Found in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas. -
Involvement in disease
Defects in SNAP29 are the cause of CEDNIK syndrome (CEDNIK) [MIM:609528]. CEDNIK is a neurocutaneous syndrome characterized by cerebral dysgenesis, neuropathy, ichthyosis and palmoplantar keratoderma. -
Sequence similarities
Belongs to the SNAP-25 family.
Contains 1 t-SNARE coiled-coil homology domain. -
Cellular localization
Cytoplasm. Membrane. Cell junction > synapse > synaptosome. Appears to be mostly membrane-bound, probably via interaction with syntaxins, but a significant portion is cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 9342 Human
- Entrez Gene: 67474 Mouse
- Entrez Gene: 116500 Rat
- Omim: 604202 Human
- SwissProt: O95721 Human
- SwissProt: Q9ERB0 Mouse
- SwissProt: Q9Z2P6 Rat
- Unigene: 108002 Human
see all -
Alternative names
- CEDNIK antibody
- FLJ21051 antibody
- SNAP 29 antibody
see all
Images
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All lanes : Anti-SNAP29 antibody [EPR9199] (ab138500) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SNAP29 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab138500 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.
ab138500 Anti-SNAP29 antibody [EPR9199] was shown to specifically react with SNAP29 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265289 (knockout cell lysate ab257693) was used. Wild-type and SNAP29 knockout samples were subjected to SDS-PAGE. ab138500 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-SNAP29 antibody [EPR9199] (ab138500) at 1/2000 dilution (purified)
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
All lanes : Anti-SNAP29 antibody [EPR9199] (ab138500) at 1/2000 dilution (purified)
Lane 1 : HeLa cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
ab138500 (purified) at 1/20 immunoprecipitating SNAP29 in 10 μg HeLa cell lysate (Lanes 1 and 2, observed at 29 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-SNAP29 antibody [EPR9199] (ab138500) at 1/1000 dilution (unpurified)
Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab138500 has been referenced in 18 publications.
- Solvik TA et al. Secretory autophagy maintains proteostasis upon lysosome inhibition. J Cell Biol 221:N/A (2022). PubMed: 35446347
- Miao G et al. ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated assembly of the SNARE complex required for autolysosome formation. Dev Cell 56:427-442.e5 (2021). PubMed: 33422265
- Cardenas RA et al. SNAP23 is essential for platelet and mast cell development and required in connective tissue mast cells for anaphylaxis. J Biol Chem 296:100268 (2021). PubMed: 33837726
- Saha B et al. A non-canonical role for the autophagy machinery in anti-retroviral signaling mediated by TRIM5a. PLoS Pathog 16:e1009017 (2020). PubMed: 33052966
- Mailler E et al. The autophagy protein ATG9A promotes HIV-1 infectivity. Retrovirology 16:18 (2019). PubMed: 31269971