Recombinant Anti-SNAP29 antibody [EPR9199] - BSA and Azide free (ab240110)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9199] to SNAP29 - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-SNAP29 antibody [EPR9199] - BSA and Azide free
See all SNAP29 primary antibodies -
Description
Rabbit monoclonal [EPR9199] to SNAP29 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa and HepG2 cell lysates. IP: HeLa cell lysate.
-
General notes
ab240110 is the carrier-free version of ab138500.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.30 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9199 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 27 kDa.
|
|
IP |
Use at an assay dependent concentration.
|
Notes |
---|
WB
Use at an assay dependent concentration. Predicted molecular weight: 27 kDa. |
IP
Use at an assay dependent concentration. |
Target
-
Function
Involved in multiple membrane trafficking steps. -
Tissue specificity
Found in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas. -
Involvement in disease
Defects in SNAP29 are the cause of CEDNIK syndrome (CEDNIK) [MIM:609528]. CEDNIK is a neurocutaneous syndrome characterized by cerebral dysgenesis, neuropathy, ichthyosis and palmoplantar keratoderma. -
Sequence similarities
Belongs to the SNAP-25 family.
Contains 1 t-SNARE coiled-coil homology domain. -
Cellular localization
Cytoplasm. Membrane. Cell junction > synapse > synaptosome. Appears to be mostly membrane-bound, probably via interaction with syntaxins, but a significant portion is cytoplasmic. - Information by UniProt
-
Database links
- Entrez Gene: 9342 Human
- Entrez Gene: 67474 Mouse
- Entrez Gene: 116500 Rat
- Omim: 604202 Human
- SwissProt: O95721 Human
- SwissProt: Q9ERB0 Mouse
- SwissProt: Q9Z2P6 Rat
- Unigene: 108002 Human
see all -
Alternative names
- CEDNIK antibody
- FLJ21051 antibody
- SNAP 29 antibody
see all
Images
-
All lanes : Anti-SNAP29 antibody [EPR9199] (ab138500) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SNAP29 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab138500).
Lanes 1-3: Merged signal (red and green). Green - ab138500 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.
ab138500 Anti-SNAP29 antibody [EPR9199] was shown to specifically react with SNAP29 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265289 (knockout cell lysate ab257693) was used. Wild-type and SNAP29 knockout samples were subjected to SDS-PAGE. ab138500 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
ab138500 (purified) at 1/20 immunoprecipitating SNAP29 in 10 µg HeLa cell lysate (Lanes 1 and 2, observed at 29 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138500).
-
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138500).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab240110 has not yet been referenced specifically in any publications.