Recombinant Anti-SNAPC1 antibody [EPR16466] (ab197015)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16466] to SNAPC1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SNAPC1 antibody [EPR16466]
See all SNAPC1 primary antibodies -
Description
Rabbit monoclonal [EPR16466] to SNAPC1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human testis and fetal brain lysates; HeLa cell lysate; HeLa cells; U-87 MG cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16466 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/60.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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ICC/IF |
1/250.
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Notes |
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Flow Cyt (Intra)
1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
ICC/IF
1/250. |
Target
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Function
Part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes. Binds to the proximal sequence element (PSE), a non-TATA-box basal promoter element common to these 2 types of genes. Recruits TBP and BRF2 to the U6 snRNA TATA box. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6617 Human
- Omim: 600591 Human
- SwissProt: Q16533 Human
- Unigene: 179312 Human
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Alternative names
- Proximal sequence element-binding transcription factor subunit gamma antibody
- PSE binding factor subunit gamma antibody
- PSE-binding factor subunit gamma antibody
see all
Images
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All lanes : Anti-SNAPC1 antibody [EPR16466] (ab197015) at 1/5000 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-SNAPC1 antibody [EPR16466] (ab197015) at 1/5000 dilution + Human fetal brain tissue lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling SNAPC1 with ab197015 at 1/250 dilution, followed by Alexa Fluor®488 Goat Anti-Rabbit IgG H&L (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab197015 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
Intracellular flow cytometric analysis ofU-87 MG (Human glioblastoma-astrocytoma epithelial cell line)cells labeling SNAPC1with ab197015 at 1/60 dilution (red)compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab197015 has not yet been referenced specifically in any publications.