Overview

  • Product name

    Anti-SNAPC1 antibody [EPR16466]
    See all SNAPC1 primary antibodies
  • Description

    Rabbit monoclonal [EPR16466] to SNAPC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SNAPC1 aa 1-100. The exact sequence is proprietary.
    Database link: Q16533

  • Positive control

    • Human testis and fetal brain lysates; HeLa cell lysate; HeLa cells; U-87 MG cells.
  • General notes

     

     

     This product was previously labelled as SNAP43

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab197015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
ICC/IF 1/250.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes. Binds to the proximal sequence element (PSE), a non-TATA-box basal promoter element common to these 2 types of genes. Recruits TBP and BRF2 to the U6 snRNA TATA box.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Proximal sequence element-binding transcription factor subunit gamma antibody
    • PSE binding factor subunit gamma antibody
    • PSE-binding factor subunit gamma antibody
    • PTF subunit gamma antibody
    • PTFgamma antibody
    • Small nuclear RNA activating complex polypeptide 1 antibody
    • small nuclear RNA activating complex, polypeptide 1, 43kDa antibody
    • Small nuclear RNA-activating complex polypeptide 1 antibody
    • Small nuclear RNA-activating protein complex, 43-KD subunit antibody
    • SNAP43 antibody
    • SNAPc 43 kDa subunit antibody
    • SNAPc subunit 1 antibody
    • Snapc1 antibody
    • SNPC1_HUMAN antibody
    • snRNA activating protein complex 43 kDa subunit antibody
    • snRNA activating protein complex subunit 1 antibody
    • snRNA-activating protein complex 43 kDa subunit antibody
    • snRNA-activating protein complex subunit 1 antibody
    see all

Images

  • All lanes : Anti-SNAPC1 antibody [EPR16466] (ab197015) at 1/5000 dilution

    Lane 1 : Human testis lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-SNAPC1 antibody [EPR16466] (ab197015) at 1/5000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling SNAPC1 with ab197015 at 1/250 dilution, followed by Alexa Fluor®488 Goat Anti-Rabbit IgG H&L (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab197015 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Flow cytometric analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling SNAPC1 with ab197015 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

References

ab197015 has not yet been referenced specifically in any publications.

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