Key features and details
- Chicken polyclonal to SNAPIN
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgY
Product nameAnti-SNAPIN antibody
See all SNAPIN primary antibodies
DescriptionChicken polyclonal to SNAPIN
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide, corresponding to amino acids 31-136 of Human SNAPIN
- Brain tissue
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab37496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 70 kDa for the fusion protein (predicted molecular weight for the native protein: 15 kDa).|
FunctionMay modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release by potentiating the interaction of synaptotagmins with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells.
Tissue specificityExpressed in male germ cells of adult testis (at protein level).
Sequence similaritiesBelongs to the SNAPIN family.
Developmental stageExpressed in germ cells of 22-week prenatal testis.
Cellular localizationCytoplasm. Membrane. Cytoplasmic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Cytoplasm > perinuclear region. May be cytoplasmic and peripheral membrane bound or anchored to the vesicular membrane through an N-terminal signal anchor (By similarity). Co-localizes with NANOS1 and PUM2 in the perinuclear region of germ cells.
- Information by UniProt
- OTTHUMP00000035157 antibody
- SNAP associated protein antibody
- SNAP-25-binding protein antibody
Anti-SNAPIN antibody (ab37496) at 1/2000 dilution + E. coli derived fusion protein at 2.5 µg
Goat anti IgY HRP at 1/1000 dilution
Observed band size: 70 kDa why is the actual band size different from the predicted?
Band size larger than expected as a fusion protein was used as positive control.
ab37496 has been referenced in 3 publications.
- Zhang Q et al. E3 ubiquitin ligase RNF13 involves spatial learning and assembly of the SNARE complex. Cell Mol Life Sci 70:153-65 (2013). PubMed: 22890573
- Blandin G et al. A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome. Skelet Muscle 3:3 (2013). WB . PubMed: 23414517
- Ginter-Matuszewska B et al. The SNARE-associated component SNAPIN binds PUMILIO2 and NANOS1 proteins in human male germ cells. Mol Hum Reprod 15:173-9 (2009). PubMed: 19168546