Key features and details
- Rabbit polyclonal to SND1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SND1 antibody
See all SND1 primary antibodies
DescriptionRabbit polyclonal to SND1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
- IHC-P: Human pancreas, placenta, stomach and cerebral cortex tissue. WB: SK-BR-3, U-87 MG and EFO-21 cell lysates. ICC/IF: A431 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 102 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionFunctions as a bridging factor between STAT6 and the basal transcription factor. Plays a role in PIM1 regulation of MYB activity. Functions as a transcriptional coactivator for the Epstein-Barr virus nuclear antigen 2 (EBNA2).
Tissue specificityUbiquitously expressed.
Sequence similaritiesContains 4 TNase-like domains.
Contains 1 Tudor domain.
modificationsPhosphorylated by PIM1 in vitro.
Cellular localizationCytoplasm. Nucleus. Melanosome. In IL-4 stimulated cells colocalizes with STAT6 in the nucleus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 100 kDa coactivator antibody
- EBNA 2 co activator (100kD) antibody
- EBNA2 coactivator p100 antibody
Anti-SND1 antibody (ab223770) at 0.4 µg/ml + SK-BR-3 cell lysate
Predicted band size: 102 kDa
Immunohistochemical analysis of human cerebral cortex tissue labeling SND1 with ab223770 at 1/200 dilution.
All lanes : Anti-SND1 antibody (ab223770) at 0.4 µg/ml
Lane 1 : U-87 MG cell lysate
Lane 2 : EFO-21 cell lysate
Predicted band size: 102 kDa
Immunohistochemical analysis of human stomach tissue labeling SND1 with ab223770 at 1/200 dilution.
Immunohistochemical analysis of human placenta tissue labeling SND1 with ab223770 at 1/200 dilution.
Immunohistochemical analysis of human pancreas tissue labeling SND1 with ab223770 at 1/200 dilution.
Immunohistochemical analysis of human skeletal muscle tissue labeling SND1 with ab223770 at 1/200 dilution. No staining is observed.
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SND1 (green) using ab223770 at 4 µg/ml in ICC/IF.
ab223770 has been referenced in 1 publication.
- Yao X et al. Protective effects of SND1 in retinal photoreceptor cell damage induced by ionizing radiation. Biochem Biophys Res Commun 514:919-925 (2019). PubMed: 31084926