Overview

  • Product name

  • Description

    Rabbit polyclonal to SND1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Orangutan
  • Immunogen

    Synthetic peptide within Human SND1 aa 100-150. The exact sequence is proprietary. (NP_055205).
    Database link: Q7KZF4

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate (ab150035). IHC-P: Human lung carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab225620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 102 kDa.

Target

  • Function

    Functions as a bridging factor between STAT6 and the basal transcription factor. Plays a role in PIM1 regulation of MYB activity. Functions as a transcriptional coactivator for the Epstein-Barr virus nuclear antigen 2 (EBNA2).
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Contains 4 TNase-like domains.
    Contains 1 Tudor domain.
  • Post-translational
    modifications

    Phosphorylated by PIM1 in vitro.
  • Cellular localization

    Cytoplasm. Nucleus. Melanosome. In IL-4 stimulated cells colocalizes with STAT6 in the nucleus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • 100 kDa coactivator antibody
    • EBNA 2 co activator (100kD) antibody
    • EBNA2 coactivator p100 antibody
    • p100 antibody
    • p100 co-activator antibody
    • p100 coactivator antibody
    • p100 EBNA2 co activator antibody
    • SND 1 antibody
    • Snd1 antibody
    • SND1_HUMAN antibody
    • Staphylococcal nuclease and tudor domain containing 1 antibody
    • Staphylococcal nuclease domain containing 1 antibody
    • Staphylococcal nuclease domain containing protein 1 antibody
    • Staphylococcal nuclease domain-containing protein 1 antibody
    • TDRD11 antibody
    • TRD 11 antibody
    • TRD11 antibody
    • Tudor domain containing protein 11 antibody
    • Tudor domain-containing protein 11 antibody
    see all

Images

  • All lanes : Anti-SND1 antibody (ab225620) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 102 kDa


    Exposure time: 30 seconds
  • Formalin fixed, paraffin embedded human lung carcinoma tissue stained for SND1 using ab225620 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.

  • SND1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225620 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225620 at 0.4 µg/ml.

    Lane 1: ab225620 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 10 seconds.

References

ab225620 has not yet been referenced specifically in any publications.

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