Anti-SNF2H antibody (ab168653)
Key features and details
- Mouse polyclonal to SNF2H
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SNF2H antibody
See all SNF2H primary antibodies -
Description
Mouse polyclonal to SNF2H -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Full length protein corresponding to amino acids 1-1052 of Human SNF2H (AAH23144.1).
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Positive control
- SNF2H transfected 293T cell lysate and Human liver tissue lysate; HeLa cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab168653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 122 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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Function
Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 SANT domains. -
Developmental stage
Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8467 Human
- Entrez Gene: 93762 Mouse
- Omim: 603375 Human
- SwissProt: O60264 Human
- SwissProt: Q91ZW3 Mouse
- Unigene: 558422 Human
- Unigene: 246803 Mouse
- Unigene: 474173 Mouse
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Alternative names
- 4933427E24Rik antibody
- D030040M08Rik antibody
- D330027N15Rik antibody
see all
Images
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Western blot - Anti-SNF2H antibody (ab168653)All lanes : Anti-SNF2H antibody (ab168653) at 1 µg/ml
Lane 1 : SNF2H transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP
at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 122 kDa -
Western blot - Anti-SNF2H antibody (ab168653)Anti-SNF2H antibody (ab168653) at 1/500 dilution + Human liver tissue lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP
at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 122 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-SNF2H antibody (ab168653)Immunofluorescence analysis of HeLa cells, labeling SNF2H with ab168653 at 10 µg/ml.
Protocols
Datasheets and documents
References (0)
ab168653 has not yet been referenced specifically in any publications.
