Anti-SNF2L antibody (ab72287)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-SNF2L antibody
See all SNF2L primary antibodies -
Description
Rabbit polyclonal to SNF2L -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Rhesus monkey, Gorilla, Orangutan, Elephant
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Immunogen
Synthetic peptide corresponding to Human SNF2L aa 1004-1054.
Database link: NP_003060.2 -
Positive control
- HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab72287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IP | Use at 2-5 µg/mg of lysate. | |
| WB | 1/2000 - 1/10000. Detects a band of approximately 123 kDa (predicted molecular weight: 123 kDa). Not recommended for blotting crude sources of SNF2L. ab72287 can be used for blotting enriched sources (e.g. immunoprecipitated and exogenously expressed) of SNF2L. |
Target
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Relevance
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. Two transcript variants encoding different isoforms have been found for this gene. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 6594 Human
- Entrez Gene: 93761 Mouse
- Entrez Gene: 317575 Rat
- Omim: 300012 Human
- SwissProt: P28370 Human
- SwissProt: Q6PGB8 Mouse
- Unigene: 152292 Human
- Unigene: 229151 Mouse
see all -
Alternative names
- ATP dependent helicase SMARCA1 antibody
- global transcription activator homologous sequence antibody
- ISWI antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF2L antibody (ab72287)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SNF2L with ab72287 at 1/5000 (0.2µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-SNF2L antibody (ab72287)Detection of Human SNF2L by Immunoprecipitation from HeLa whole cell lysate, (1 mg for IP, 20% of IP loaded) using ab72287 at 3µg/mg lysate for IP (Lane 1) and 1µg/ml for subsequent WB. Lane 2 represents control IgG IP.
References
ab72287 has not yet been referenced specifically in any publications.
