Anti-SNF2L antibody (ab72287)
Key features and details
- Rabbit polyclonal to SNF2L
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SNF2L antibody
See all SNF2L primary antibodies -
Description
Rabbit polyclonal to SNF2L -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Rhesus monkey, Gorilla, Orangutan, Elephant
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Immunogen
Synthetic peptide corresponding to Human SNF2L aa 1004-1054.
Database link: NP_003060.2 -
Positive control
- HeLa whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab72287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IP | Use at 2-5 µg/mg of lysate. |
Target
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Relevance
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. Two transcript variants encoding different isoforms have been found for this gene. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 6594 Human
- Entrez Gene: 93761 Mouse
- Entrez Gene: 317575 Rat
- Omim: 300012 Human
- SwissProt: P28370 Human
- SwissProt: Q6PGB8 Mouse
- Unigene: 152292 Human
- Unigene: 229151 Mouse
see all -
Alternative names
- ATP dependent helicase SMARCA1 antibody
- global transcription activator homologous sequence antibody
- ISWI antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF2L antibody (ab72287)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SNF2L with ab72287 at 1/5000 (0.2µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-SNF2L antibody (ab72287)Detection of Human SNF2L by Immunoprecipitation from HeLa whole cell lysate, (1 mg for IP, 20% of IP loaded) using ab72287 at 3µg/mg lysate for IP (Lane 1) and 1µg/ml for subsequent WB. Lane 2 represents control IgG IP.
Protocols
References (0)
ab72287 has not yet been referenced specifically in any publications.
