Overview

  • Product name

    Anti-SNF5/SMARCB1 antibody [EPR6966]
    See all SNF5/SMARCB1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6966] to SNF5/SMARCB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Zebrafish
  • Immunogen

    Synthetic peptide within Human SNF5/SMARCB1 aa 350-450 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, Jurkat, K562, and 293T cell lysates.
  • General notes

     

     

     This product was previously labelled as SNF5

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
    • Involvement in disease

      Defects in SMARCB1 are a cause of rhabdoid tumor (RDT) [MIM:609322]; also known as malignant rhabdoid tumor (MRT). RDT are a highly malignant group of neoplasms that usually occur in early childhood. SMARCB1/INI1 is also frequently inactivated in epithelioid sarcomas.
      Defects in SMARCB1 are a cause of schwannomatosis (SCHWA) [MIM:162091]; also called congenital cutaneous neurilemmomatosis. Schwannomas are benign tumors of the peripheral nerve sheath that usually occur singly in otherwise normal individuals. Multiple schwannomas in the same individual suggest an underlying tumor-predisposition syndrome. The most common such syndrome is NF2. The hallmark of NF2 is the development of bilateral vestibular-nerve schwannomas; but two-thirds or more of all NF2-affected individuals develop schwannomas in other locations, and dermal schwannomas may precede vestibular tumors in NF2-affected children. There have been several reports of individuals with multiple schwannomas who do not show evidence of vestibular schwannoma. Clinical report suggests that schwannomatosis is a clinical entity distinct from other forms of neurofibromatosis.
    • Sequence similarities

      Belongs to the SNF5 family.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • BAF47 antibody
      • BRG1-associated factor 47 antibody
      • hSNF5 antibody
      • INI1 antibody
      • Integrase interactor 1 protein antibody
      • Malignant rhabdoid tumor suppressor antibody
      • RDT antibody
      • RTPS1 antibody
      • Sfh1p antibody
      • SMARCB1 antibody
      • SNF5 homolog antibody
      • SNF5_HUMAN antibody
      • SNF5L1 antibody
      • Snr1 antibody
      • Sucrose nonfermenting yeast homolog like 1 antibody
      • SWI/SNF complex component SNF5 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 antibody
      • SWI10 antibody
      • Transcription factor TYE4 antibody
      • Transcription regulatory protein SNF5 antibody
      • TYE4 antibody
      see all

    Images

    • All lanes : Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 44 kDa

    • Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734) at 1/2000 dilution + Zebrafish whole mount purified protein at 15 µg

      Secondary
      Undiluted HRP-conjugated goat anti-rabbit IgG polyclonal

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 44 kDa
      Observed band size: 44 kDa
      Additional bands at: 15 kDa (possible non-specific binding), 28 kDa (possible non-specific binding)


      Exposure time: 15 seconds

      See Abreview

    References

    This product has been referenced in:

    • Castillo-Robles J  et al. smarce1 mutants have a defective endocardium and an increased expression of cardiac transcription factors in zebrafish. Sci Rep 8:15369 (2018). Read more (PubMed: 30337622) »
    • McAndrew MJ  et al. Chromatin Remodeler Recruitment during Macrophage Differentiation Facilitates Transcription Factor Binding to Enhancers in Mature Cells. J Biol Chem 291:18058-71 (2016). ChIP ; Mouse . Read more (PubMed: 27382057) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Western blot
    Sample
    Zebrafish Purified protein (whole mount)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    15 µg
    Specification
    whole mount
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Jun 23 2014

    Application
    Western blot
    Sample
    Drosophila melanogaster Tissue lysate - whole (whole adult fly or larvae)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    61 µg
    Specification
    whole adult fly or larvae
    Blocking step
    (agent) for 30 minute(s) · Concentration: 5% · Temperature: 22°C

    Miss. Kristin Eikmeier

    Verified customer

    Submitted Sep 26 2012

    Answer

    Vielen Dank für Ihre Email.

    Für die Bestellung des zu testenden Antikörpers (ab126734) brauchen Sie den Code nicht anzugeben.

    Bitte geben Sie diesen In den "additional notes" in dem Abreview an. Der Code wird damit aktiv.

    Als Dankeschön für das Testen von ab126734können Sie nach einreichen des Abreviews den Wert von ab126734 bei einer Bestellung verrechnen lassen. Dazu geben Sie bitte unbedingt den Code auf der Bestellung mit an.

    D.h. dass der zu testende Antikörper ganz normal gekauft wird, während der Wert des getesteten Antikörpers nach der Einsendung der Ergebnisse als Gutschein verwendet werden kann für alle Produkte aus unserem Katalog.

    Ich hoffe, diese Information ist hilfreich. Bitte lassen Sie mich wissen, falls noch Unklarheiten bestehen, ich bin mir bewusst, dass dieses System ein bisschen verwirrend ist.

    Read More

    Answer

    DISCOUNT CODE: 100%ABR-000000

    Ablaufdatum: 07. December 2012

    Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab126734 in Drosophila zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer xxxx, deckt den Wert von ab126734 ab und kann zum Kauf weiterer Produkte eingesetzt werden.

    Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in Drosophilaeingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

    Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

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