Recombinant Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734)
![Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734)](https://www.abcam.com/ps/products/126/ab126734/Images/ab126734-393637-anti-snf5smarcb1-antibody-epr6966-western-blot-human-knockout-lysate.jpg "Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6966] to SNF5/SMARCB1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-SNF5/SMARCB1 antibody [EPR6966]
See all SNF5/SMARCB1 primary antibodies -
Description
Rabbit monoclonal [EPR6966] to SNF5/SMARCB1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Zebrafish
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Jurkat, K562, Daudi and HEK293T cell lysates.
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General notes
This product was previously labelled as SNF5
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6966 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Target
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Function
Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. -
Involvement in disease
Defects in SMARCB1 are a cause of rhabdoid tumor (RDT) [MIM:609322]; also known as malignant rhabdoid tumor (MRT). RDT are a highly malignant group of neoplasms that usually occur in early childhood. SMARCB1/INI1 is also frequently inactivated in epithelioid sarcomas.
Defects in SMARCB1 are a cause of schwannomatosis (SCHWA) [MIM:162091]; also called congenital cutaneous neurilemmomatosis. Schwannomas are benign tumors of the peripheral nerve sheath that usually occur singly in otherwise normal individuals. Multiple schwannomas in the same individual suggest an underlying tumor-predisposition syndrome. The most common such syndrome is NF2. The hallmark of NF2 is the development of bilateral vestibular-nerve schwannomas; but two-thirds or more of all NF2-affected individuals develop schwannomas in other locations, and dermal schwannomas may precede vestibular tumors in NF2-affected children. There have been several reports of individuals with multiple schwannomas who do not show evidence of vestibular schwannoma. Clinical report suggests that schwannomatosis is a clinical entity distinct from other forms of neurofibromatosis. -
Sequence similarities
Belongs to the SNF5 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6598 Human
- Entrez Gene: 20587 Mouse
- Entrez Gene: 361825 Rat
- Omim: 601607 Human
- SwissProt: Q12824 Human
- SwissProt: Q9Z0H3 Mouse
- SwissProt: Q5U379 Zebrafish
- Unigene: 534350 Human
see all -
Alternative names
- BAF47 antibody
- BRG1-associated factor 47 antibody
- hSNF5 antibody
see all
Images
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Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734)All lanes : Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : SMARCB1 knockout HEK293T cell lysate
Lane 3 : Daudi cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 44 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab126734 observed at 50 kDa. Red - loading control ab8245 observed at 36 kDa.
ab126734 Anti-SNF5/SMARCB1 antibody [EPR6966] was shown to specifically react with SNF5/SMARCB1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267269 (knockout cell lysate ab257688) was used. Wild-type and SNF5/SMARCB1 knockout samples were subjected to SDS-PAGE. ab126734 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734)](https://www.abcam.com/ps/products/126/ab126734/Images/ab126734-140714-anti-snf5-antibody-epr6966-western-blot.jpg)
Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734)All lanes : Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 44 kDa -
![Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734)](https://www.abcam.com/ps/products/126/ab126734/Images/ab126734-228395-anti-snf5-antibody-epr6966-western-blot.jpg)
Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734)This image is courtesy of an anonymous AbreviewAnti-SNF5/SMARCB1 antibody [EPR6966] (ab126734) at 1/2000 dilution + Zebrafish whole mount purified protein at 15 µg
Secondary
Undiluted HRP-conjugated goat anti-rabbit IgG polyclonal
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 15 kDa (possible non-specific binding), 28 kDa (possible non-specific binding)
Exposure time: 15 seconds
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![Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734)](https://www.abcam.com/ps/products/126/ab126734/Images/ab126734-4-benefits-of-recombinant-antibodies.png)
Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734)
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab126734 has been referenced in 4 publications.
- Schick S et al. Systematic characterization of BAF mutations provides insights into intracomplex synthetic lethalities in human cancers. Nat Genet 51:1399-1410 (2019). PubMed: 31427792
- Castillo-Robles J et al. smarce1 mutants have a defective endocardium and an increased expression of cardiac transcription factors in zebrafish. Sci Rep 8:15369 (2018). PubMed: 30337622
- McAndrew MJ et al. Chromatin Remodeler Recruitment during Macrophage Differentiation Facilitates Transcription Factor Binding to Enhancers in Mature Cells. J Biol Chem 291:18058-71 (2016). ChIP ; Mouse . PubMed: 27382057
- Giessrigl B et al. Fulvestrant induces resistance by modulating GPER and CDK6 expression: implication of methyltransferases, deacetylases and the hSWI/SNF chromatin remodelling complex. Br J Cancer 109:2751-62 (2013). Human . PubMed: 24169358