Anti-SNIP1 antibody (ab126194)
Key features and details
- Rabbit polyclonal to SNIP1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SNIP1 antibody
See all SNIP1 primary antibodies -
Description
Rabbit polyclonal to SNIP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 41-323 of Human SNIP1 (Q8TAD8).
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Positive control
- Human 59T xenograft, Rat RT2 xenograft and Mouse Muscle tissues; 293T, A431, Jurkat and Raji whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 46 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Down-regulates NF-kappa-B signaling by competing with RELA for CREBBP/EP300 binding. Involved in the microRNA (miRNA) biogenesis. -
Tissue specificity
Ubiquitous, with highest expression in heart and skeletal muscle. -
Sequence similarities
Contains 1 FHA domain. -
Post-translational
modificationsDegraded by the proteasome upon binding to the SMAD1/OAZ1/PSMB4 complex.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 79753 Human
- Entrez Gene: 76793 Mouse
- Entrez Gene: 313588 Rat
- Omim: 608241 Human
- SwissProt: Q8TAD8 Human
- SwissProt: Q8BIZ6 Mouse
- SwissProt: Q5M9G6 Rat
- Unigene: 471951 Human
see all -
Alternative names
- FHA domain containing protein SNIP1 antibody
- FHA domain-containing protein SNIP1 antibody
- FLJ12553 antibody
see all
Images
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Anti-SNIP1 antibody (ab126194) at 1/5000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 46 kDa
10% SDS PAGE -
ab126194, at 1/500 dilution, staining SNIP1 in Paraffin-embedded Human 59T xenograft tissue by Immunohistochemistry.
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ab126194, at 1/500 dilution, staining SNIP1 in Paraffin-embedded HumRat RT2 xenograft tissue by Immunohistochemistry.
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ab126194, at 1/500 dilution, staining SNIP1 in Paraffin-embedded Mouse Muscle tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab126194 has been referenced in 2 publications.
- Nakamura K et al. Functional analysis of the 1p34.3 risk locus implicates GNL2 in high-grade serous ovarian cancer. Am J Hum Genet 109:116-135 (2022). PubMed: 34965383
- Yu B et al. KMT5A-methylated SNIP1 promotes triple-negative breast cancer metastasis by activating YAP signaling. Nat Commun 13:2192 (2022). PubMed: 35449131