Key features and details
- Rabbit polyclonal to SNM1A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SNM1A antibody
See all SNM1A primary antibodies
DescriptionRabbit polyclonal to SNM1A
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Gorilla
Synthetic peptide within Human SNM1A aa 425-475. The exact sequence is proprietary. (NP_055696.3).
Database link: Q6PJP8
- WB: HEK-293T, HeLa and Jurkat whole cell lysate (ab7899). IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226003 was affinity purified using an epitope specific to SNM1A immobilized on solid support
Our Abpromise guarantee covers the use of ab226003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 116 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay be required for DNA interstrand cross-link repair. Also required for checkpoint mediated cell cycle arrest in early prophase in response to mitotic spindle poisons.
Tissue specificityExpressed in brain, heart, kidney, liver, pancreas, placenta and skeletal muscle.
Sequence similaritiesBelongs to the DNA repair metallo-beta-lactamase (DRMBL) family.
Cellular localizationNucleus. In some cells it may be found in typically 1 or 2 discrete nuclear aggregates of unknown function which also contain TP53BP1. Also found in multiple discrete nuclear foci which increase in number following treatment with ionizing radiation or interstrand cross-linking agents. These foci overlap with those formed by the MRN complex (composed of MRE11A, RAD50 and NBN) and BRCA1.
- Information by UniProt
- Dclre1a antibody
- DCR1A_HUMAN antibody
- DNA cross link repair 1A antibody
All lanes : Anti-SNM1A antibody (ab226003) at 0.1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 116 kDa
Exposure time: 3 minutes
SNM1A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226003 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226003 at 1 µg/ml.
Lane 1: ab226003 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226003 has not yet been referenced specifically in any publications.