Overview

  • Product name

  • Description

    Rabbit polyclonal to SNM1A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Gorilla
  • Immunogen

    Synthetic peptide within Human SNM1A aa 425-475. The exact sequence is proprietary. (NP_055696.3).
    Database link: Q6PJP8

  • Positive control

    • WB: HEK-293T, HeLa and Jurkat whole cell lysate (ab7899). IP: HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226003 was affinity purified using an epitope specific to SNM1A immobilized on solid support
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 116 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    May be required for DNA interstrand cross-link repair. Also required for checkpoint mediated cell cycle arrest in early prophase in response to mitotic spindle poisons.
  • Tissue specificity

    Expressed in brain, heart, kidney, liver, pancreas, placenta and skeletal muscle.
  • Sequence similarities

    Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.
  • Cellular localization

    Nucleus. In some cells it may be found in typically 1 or 2 discrete nuclear aggregates of unknown function which also contain TP53BP1. Also found in multiple discrete nuclear foci which increase in number following treatment with ionizing radiation or interstrand cross-linking agents. These foci overlap with those formed by the MRN complex (composed of MRE11A, RAD50 and NBN) and BRCA1.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dclre1a antibody
    • DCR1A_HUMAN antibody
    • DNA cross link repair 1A antibody
    • DNA cross link repair gene SNM1 antibody
    • DNA cross-link repair 1A protein antibody
    • hSNM1 antibody
    • hSNM1A antibody
    • PSO2 antibody
    • SNM1 homolog A antibody
    see all

Images

  • All lanes : Anti-SNM1A antibody (ab226003) at 0.1 µg/ml

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 2 : HEK-293T whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 116 kDa


    Exposure time: 3 minutes
  • SNM1A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226003 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226003 at 1 µg/ml.

    Lane 1: ab226003 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab226003 has not yet been referenced specifically in any publications.

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